This pcDNA™3.1(-) vector is designed for high-level, constitutive expression in a variety of mammalian cell lines. It contains a Geneticin™ selectable marker and a reverse-orientation multiple cloning site.
The pcDNA™3.1 Expression Vector Family Three untagged versions of pcDNA™3.1 (available separately), each with a different selectable marker (Geneticin™, Zeocin™, or Hygromycin), are for use alone or in co-transfections. All three vectors offer the following features: • Cytomegalovirus (CMV) enhancer-promoter for high-level expression • Large multiple cloning site in either forward (+) or reverse (-) orientations • Bovine Growth Hormone (BGH) polyadenylation signal and transcription termination sequence for enhanced mRNA stability • SV40 origin for episomal replication and simple vector rescue in cell lines expressing the large T antigen (i.e., COS-1 and COS-7) • Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli
For Research Use Only. Not for use in diagnostic procedures.
規格
Delivery Type
Transfection
Promoter
CMV
Product Type
Mammalian Expression Vector
Selection Agent (Eukaryotic)
Geneticin™ (G-418)
Protein Tag
Untagged
Cloning Method
Restriction Enzyme/MCS
Quantity
20 μg
Vector
pcDNA
Product Line
pcDNA™
For Use With (Application)
Constitutive Expression
Unit Size
20 µg
內容物與存放
20 μg of this pcDNA™3.1(-) vector, as well as an expression control, are supplied supercoiled and lyophilized. Store at -20°C. Vectors are guaranteed stable for 6 months when properly stored.