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SuperScript™ VILO™ Master Mix
Upgrade to SuperScript IV VILO Master Mix for superior performance
SuperScript™ VILO™ Master Mix
Invitrogen™

SuperScript™ VILO™ Master Mix

SuperScript VILO Master Mix is a first-strand cDNA synthesis master mix for two-step RT-qPCR applications. The master mix combines SuperScriptRead more
Catalog NumberNo. of Reactions
11755500500 Reactions
1175505050 Reactions
11755250250 Reactions
Catalog number 11755500
Price (JPY)
452,200
Each
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No. of Reactions:
500 Reactions
Request bulk or custom format

SuperScript VILO Master Mix is a first-strand cDNA synthesis master mix for two-step RT-qPCR applications. The master mix combines SuperScript III Reverse Transcriptase (RT) and other RT reaction components in a single-tube format to enable reliable first-strand synthesis and high cDNA yields from a wide range of input RNA concentrations. The high RT efficiency of SuperScript III RT and the optimized formulation allow the master mix to deliver high linearity in cDNA synthesis across a broad range of input RNA template for two-step quantitative RT-PCR assays.

Features of SuperScript VILO Master Mix include:
• Starting material can range up to 2.5 μg of total RNA in a 20 μL cDNA synthesis reaction
• Recombinant ribonuclease inhibitor safeguards against the degradation of target RNA due to ribonuclease contamination
• RT is engineered with reduced RNase H activity and increased thermal stability

For superior cDNA synthesis performance in RT-qPCR applications
SuperScript IV VILO Master Mix elevates the trusted VILO technology to the next level with the highly processive and thermostable SuperScript IV RT and further optimized buffer. The master mix enables efficient cDNA synthesis at higher temperatures in less time and provides superior cDNA yield and sensitivity even with suboptimal purity or scarce templates.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Concentration5X
Final Product TypeFirst-Strand cDNA
FormatMaster Mix
No. of Reactions500 Reactions
Optimal Reaction Temperature42°C
Quantity500 reactions
Reaction FormatMaster Mix
Reagent TypeReverse Transcription
Reverse TranscriptaseSuperScript III
Ribonuclease H ActivityReduced
Shipping ConditionDry Ice
Size (Final Product)Up to 10 kb
Starting MaterialRNA
TechniqueReverse Transcription
Detection MethodqPCR
For Use With (Application)Real Time PCR (qPCR)
GC-Rich PCR PerformanceHigh
Reaction Speed60 min.
Unit SizeEach
Contents & Storage

• SuperScript VILO Master Mix (2 mL)

Store at –5 to –30°C.

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