Thermo Scientific Pierce Sulfo-EMCS is a water-soluble amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends ofRead more
Thermo Scientific Pierce Sulfo-EMCS is a water-soluble amine-to-sulfhydryl crosslinker that contains NHS-ester and maleimide reactive groups at opposite ends of a medium-length spacer arm (9.4 angstroms).
Features of Sulfo-EMCS:
• Reactive groups: sulfo-NHS ester and maleimide • Reactive towards: amino and sulfhydryl groups • Sulfo-NHS ester end couples with primary amines at pH 7-9 to form stable amide bonds • Maleimide reacts with -SH groups at pH 6.5-7.5, forming stable thioether linkages • Non-cleavable • Water-soluble (compare to EMCS) • Aliphatic spacer offers low potential for eliciting an immune response
Product References: Crosslinker Application Guide -- search for recent literature references for this product