SuperBlock™ Blocking Buffer
SuperBlock™ Blocking Buffer
Thermo Scientific™

SuperBlock™ Blocking Buffer

SuperBlock Blocking Buffer is an optimized solution containing a single purified glycoprotein that provides incredibly fast and effective blocking for ELISA, IHC, and western blot analysis.
Catalog NumberQuantityProduct TypeBuffer
375151 LBlocking BufferPBS
37580100 mLBlocking BufferPBS
375185 LBlocking BufferPBS
37581100 mLBlocking BufferTBS
375351 LBlocking BufferTBS
375455 packsBlocking Buffer, dry blendTBS
375161 LBlocking BufferPBST
375361 LBlocking BufferTBST
Catalog number 37515
Price (JPY)
47,400
Each
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Quantity:
1 L
Product Type:
Blocking Buffer
Buffer:
PBS
Request bulk or custom format
SuperBlock Blocking Buffer is an optimized solution containing a single purified glycoprotein that provides incredibly fast and effective blocking for ELISA, IHC, and western blot analysis. Blocking micro-plates, membranes, or tissues with a SuperBlock buffer yields a high signal-to-noise ratio in most detection systems.
Features of SuperBlock Blocking Buffer include:
Blocking time—block membranes in 5 to 10 minutes and ELISA plates in 2 minutes
Applications—ELISA, western blotting, IHC; biotin-free for use with streptavidin system
Stability—store buffer at 4°C for one year; store blocked plates dry for up to 12 months

Blocking micro-plates, membranes, or tissues with SuperBlock buffer yields a high signal-to-noise ratio in most detection systems. The protein-based formulation does not contain any immunoglobulins, albumin, or endogenous biotin, making it compatible in many situations where traditional blocking agents fail. The buffer is especially effective at blocking coated polystyrene microplates (96-well plates) and stabilizing them for drying and storage for later use.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
Blocking AgentSingle purified protein
BufferPBS
Concentration1X
For Use With (Application)ELISA, Western Blot
FormLiquid
Product LineSuperBlock™
Product TypeBlocking Buffer
Quantity1 L
Unit SizeEach
Contents & Storage
Upon receipt store at 4°C. Product is shipped at ambient temperature.

Frequently asked questions (FAQs)

What should I do about the colloidal carbon (black precipitate) in SuperBlock Blocking Buffer solution?

Allow any colloidal carbon, if present, to settle to the bottom of the bottle and pouring off what you need to use. We do not recommend centrifugation or filtering because these procedures may reduce the product's blocking capabilities.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What preservative is used in SuperBlock Blocking Buffer, and what is its source?

Kathon Preservative, a broad spectrum antimicrobial agent, is used at a concentration of 600 ppm.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What is SuperBlock Blocking Buffer's shelf life?

Its shelf life is one year at 4°C.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

What incubation time and temperature should I use for effective blocking using the SuperBlock Blocking Buffer ?

Typical procedures use 1 hour at room temperature or overnight at 4°C. However, sufficient blocking in many procedures is possible in 3 x 2 minutes at room temperature.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Is SuperBlock Blocking Buffer RNase-free?

No, SuperBlock Blocking Buffer is not RNase-free.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

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