The TaqMan™ Array Rodent MicroRNA A+B Cards Set v3.0 is a two card set containing a total of 384 TaqMan™ MicroRNA Assays per card. The set enables accurate quantitation of 641 and 373 unique microRNAs for mouse and rat respectively in as little as 5 hours. Included on each array is three TaqMan™ MicroRNA Assay endogenous controls for each species to aid in data normalization and one TaqMan™ MicroRNA Assay not related to rodent as a negative control. Detail on the included assays is available in the Target List file. Purchase of the Megaplex™ RT Primers, Rodent Pool Set v3.0 is required to run this array set. An additional preamplification step is enabled by Megaplex™ PreAmp Primers, Rodent Pool Set v3.0 for situations where sensitivity is of the utmost importance and/or sample is limiting.
TaqMan™ Array MicroRNA Cards provide all the advantages of TaqMan™ MicroRNA Assays in a convenient, pre-configured microfluidic card, minimizing the experimental variability and effort required to run 384 TaqMan™ MicroRNA Assays in parallel.
Megaplex™ RT Primers are highly multiplexed RT primers designed to convert up to 381 miRNAs to cDNA prior to real-time analysis, and are required to run the TaqMan™ Array MicroRNA Card. For greater sensitivity, or when working with limited sample, Megaplex™ PreAmp Primers provide an optional preamplification step prior to real-time analysis. TaqMan™ Array MicroRNA Cards and Megaplex™ Primer Pools enable a comprehensive expression profile consistent with miRBase v21 to be created in as little as 5 hours, providing the ideal miRNA profiling solution. Key product features:
Highly specific—quantitate only the biologically active mature miRNAs
• Highly streamlined workflow reduces the number of RT (reverse transcription) reactions per sample • Simplify sample handling and increase sample throughput • Coverage for human, mouse and rat species • An ideal miRNA profiling solution
Simplify Sample Handling and Increase Sample Throughput Following reverse transcription of miRNA targets using Megaplex™ RT Primers and optional preamplification using Megaplex™ PreAmp Primers, TaqMan™ Universal PCR Master Mix II is simply combined with each reaction and pipetted into each sample loading port in the TaqMan™ Array Card, simplifying sample handling and increasing sample throughput.
Provides Comprehensive Coverage of Sanger miRBase Megaplex™ RT Pools and PreAmp Primers are available for human and rodent species, and consist of pre-selected and matched content across a series of primer pools and TaqMan™ Array Cards. The content is derived from miRBase v14 and v15 for human and rodent species respectively, providing close to full coverage of Sanger with the most up-to-date TaqMan™ MicroRNA Assays.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
TypeArray Rodent MicroRNA A+B Cards Set
Format384-well Microfluidic Card
SpeciesMouse, Rat
FormDried Down
For Use With (Equipment)AB 7900 HT 384-Well System
Includes384-Well Arrays, Information CD
Label or DyeFAM
Product LineTaqMan™
Quantity4 x 2 cards
Reaction SpeedStandard
Research CategoryGene Regulation, Epigenetics
Unit Size4 x 2 cards
Contents & Storage
Contents: 384-well arrays and information CD. Storage: The TaqMan™ MicroRNA Arrays are shipped at ambient temperature. Upon arrival, store arrays at 2 to 8 ° and keep them protected from light.