NP-40 Surfact-Amps™ Detergent Solution
NP-40 Surfact-Amps™ Detergent Solution
Thermo Scientific™

NP-40 Surfact-Amps™ Detergent Solution

Thermo Scientific Surfact-Amps NP-40 Detergent Solution is highly-purified NP-40 detergent stabilized as a 10% solution in your choice of eitherRead more
Catalog NumberQuantity
8512450 mL
283246 x 10 mL
85125500 mL
Catalog number 85124
Price (JPY)
31,300
Each
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Quantity:
50 mL
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Thermo Scientific Surfact-Amps NP-40 Detergent Solution is highly-purified NP-40 detergent stabilized as a 10% solution in your choice of either 10 mL glass ampules or two sizes of HDPE-plastic bottles.

Features of Surfact-Amps NP-40 Detergent Solution:

NP-40—a nonionic detergent for use in various protein methods
Accurate—precise 10% detergent solutions in ultrapure water
Easy-to-use—solutions are simple to dispense and dilute for use
Exceptionally pure—less than 1.0μeq/mL peroxides and carbonyls
Stable—packaged under inert nitrogen gas in HDPE bottles

This is an easy-to-use 10% (w/v) solution of purified NP-40 detergent for use in routine and high-demand protein research methods and molecular biology techniques. NP-40 is a nonionic polyoxyethylene surfactant that is most frequently used as a component of cell lysis buffers or other solutions intended to extract and solubilize proteins. Surfact-Amps Detergent Solutions (10% w/v) provide unsurpassed purity, quality and stability. Unlike neat detergents, which are extremely viscous, Surfact-Amps 10% Solutions are easy to pipette and accurately dispense. The surfactant solutions are carefully prepared and packaged under nitrogen in glass ampules or non-leaching HDPE bottles, ensuring their stability and eliminating the accumulation of peroxides and degradation products.

Properties of NP-40 Detergent:
• Molecular Weight: 617g
• Detergent Class: Nonionic
• Aggregation Number: 149
• Micelle Molecular Weight: 90,000g
• Critical Micelle Concentration (CMC): 0.29 mM (0.0179%, w/v)
• Cloud Point: 80°C
• Dialyzable: No

Specifications for NP-40 Surfact-Amps Detergent Solution:
• Visual: Clear, colorless solution, free of particulate matter.
• Concentration: 10.0±1.0%
• Oxidants: ≤1.0μeq/mL
• Carbonyls: ≤1.0μeq/mL
• Suspended Solids: Residue present must not exceed Residue Reference.

Related Products
Surfact-Amps™ Detergent Sampler
For Research Use Only. Not for use in diagnostic procedures.
Specifications
FormatHDPE Bottle
Quantity50 mL
Reagent TypeDetergent Solution
FormLiquid
Product LineSurfact-Amps™
Product TypeDetergent
Unit SizeEach
Contents & Storage
Store between 20°C and 25°C
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Certificates

Lot #Certificate TypeDateCatalog Number(s)
3213291Certificate of AnalysisJun 16, 202585124
3211953Certificate of AnalysisJun 02, 202528324
3211951Certificate of AnalysisMay 21, 202585124
AC403401Certificate of AnalysisApr 02, 202585124
AA403882Certificate of AnalysisMar 14, 202528324
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Safety Data Sheets

Limited Use Label Licenses (LULL)

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Notice to Purchaser:

(OPE/NPE-containing Products for EU REACH compliance) - Applicable for uses in the EU Member States, Iceland, Liechtenstein, Norway and Switzerland

For purposes of Regulation (EC) No. 1907/2006 concerning Registration, Evaluation, Authorisation and Restriction of Chemicals (REACH), further manufacturing use of the product is limited to: 1) complete incorporation of the product into a chemical mixture(s) intended for Scientific Research and Development (SRD) uses (as defined under REACH), or 2) use of the product under controlled conditions in upstream life-cycle stages preceding SRD end-use. Further manufacturing use of the product as a manufacturing processing aid, or where controlled conditions cannot be assured, is prohibited, unless 3) an authorisation number duly issued by the European Commission to the user has been communicated and confirmed by us.

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Frequently asked questions (FAQs)

Thermo Scientific Pierce Surfact-Amps detergents are highly purified, precisely diluted (10%) formulations that are ideal for applications or assays that are sensitive to contaminants that are present in unpurified detergents. We test every batch to insure that our detergents contain less than 1.0 µeq/mL peroxides and carbonyls and package them under nitrogen, to prevent oxidization during storage.

Superior quality - lower measurable contaminant levels than other leading vendors
Accurate -precise 10% detergent solution in ultrapure water
Easy-to-use - solution is simple to dispense and dilute for use
Exceptionally pure - less than 1.0 µeq/mL peroxides and carbonyls

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Ionic detergents, or those that carry a charge, are the most likely to be denaturing to proteins. Denaturing detergents can be anionic such as sodium dodecyl sulfate (SDS) or cationic such as ethyl trimethyl ammonium bromide. These detergents totally disrupt membranes and denature proteins by breaking protein-protein interactions through changes in the three-dimensional structure of the proteins. Nondenaturing detergents can be divided into nonionic detergents (i.e., Triton X-100), bile salts (i.e., cholate), and zwitterionic detergents (i.e., CHAPS).



Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Detergents are amphipathic molecules containing both a nonpolar “tail” having aliphatic or aromatic character, and a polar “head”. The ionic character of the polar head group forms the basis for broad classification of detergents as ionic, nonionic, or zwitterionic.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Detergents are amphipathic molecules, meaning they contain both a nonpolar “tail” having aliphatic or aromatic character and a polar “head”. Like the components of biological membranes, detergents have hydrophobic-associating properties as a result of their nonpolar tail groups. Nevertheless, detergents are themselves water soluble.

Consequently, detergent molecules allow the dispersion (miscibility) of water-insoluble, hydrophobic compounds into aqueous media, including the extraction and solubilization of membrane proteins. Detergent monomers solubilize membrane proteins by partitioning into the membrane bilayer. With increasing amounts of detergents, membranes undergo various stages of solubilization.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

Detergents can be denaturing or non-denaturing with respect to protein structure. Denaturing detergents can be anionic such as sodium dodecyl sulfate (SDS) or cationic such as ethyl trimethyl ammonium bromide. These detergents totally disrupt membranes and denature proteins by breaking proteinprotein interaction. These detergents are considered harsh. Non-denaturing detergents can be divided into nonionic detergents (i.e., Triton X-100), bile salts (i.e., cholate), and zwitterionic detergents (i.e., CHAPS). These detergents do not denature proteins and do not break protein-protein interactions. These detergents are considered mild.

Find additional tips, troubleshooting help, and resources within our Protein Purification and Isolation Support Center.

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