The LanthaScreen™ TR-FRET ER alpha Competitive Binding Kit provides a sensitive and robust method for high-throughput screening (HTS) of ligands for estrogen receptor (ER) alpha. The kit uses a human ER alpha ligand-binding domain (LBD) that is tagged with glutathione S-transferase (GST), a terbium-labeled anti-GST antibody, and a fluorescent small-molecule ER ligand (Fluormone™ ES2) in a homogeneous mix-and-read assay format.
The Lanthascreen ER alpha Competitive Binding Assay is:
• Complete & ready-to-use out of the box—all reagents included, just add your test compounds • High throughput compatible—TR-FRET assays in a 384-well plate • Convenient—components available in bulk sizes for larger screening needs
For Research Use Only. Not for use in diagnostic procedures.
Specifications
For Use With (Application)
Competitive Binding Assay, Nuclear Receptor Assay
Product Line
LanthaScreen™
Quantity
800 x 20 μL assays
Type
Competitive Binding Kit
Contents & Storage
1 tube ER alpha Ligand Binding Domain Recombinant Protein (store at -68 to -85°C) 1 tube of Fluormone ES2 (store at -5 to -30°C) 2 bottles NR Buffer (store at -5 to -30°C) 1 tube Tb-Anti-GST Ab (store at -5 to -30°C) 1 tube of DTT (store at -5 to -30°C)