Collibri™ PCR-free ES DNA Library Prep Kits for Illumina Systems
Inquire about OEM or Commercial Supply version of this product here.
Collibri™ PCR-free ES DNA Library Prep Kits for Illumina Systems
Invitrogen™

Collibri™ PCR-free ES DNA Library Prep Kits for Illumina Systems

The Invitrogen Collibri PCR-free ES DNA Library Preparation kits are designed for next-generation sequencing (NGS) on Illumina systems. The kit supports whole-genome sequencing (WGS) applications and is suitable for all genomes.
Catalog NumberNo. of ReactionsProduct Type
A4360202424 PrepsPCR-free ES DNA Library Prep Kit with UD Indexes (Set B, 25-48)
A38545024W24 PrepsPCR-free ES DNA Library Prep Core Kit
A38603096W96 PrepsPCR-free ES DNA Library Prep Core Kit
A3854502424 PrepsPCR-free ES DNA Library Prep Kit with CD Indexes
A3860309696 PrepsPCR-free ES DNA Library Prep Kit with CD Indexes
A3860202424 PrepsPCR-free ES DNA Library Prep Kit with UD Indexes (Set A, 1-24)
A4360302424 PrepsPCR-free ES DNA Library Prep Kit with UD Indexes (Set C, 49-72)
A4360402424 PrepsPCR-free ES DNA Library Prep Kit with UD Indexes (Set D, 73-96)
A3860819696 PrepsPCR-free ES DNA Library Prep Kit with UD Indexes (Set A-D, 1-96)
Catalog number A43602024
Price (JPY)
138,400
Each
Contact Us ›
No. of Reactions:
24 Preps
Product Type:
PCR-free ES DNA Library Prep Kit with UD Indexes (Set B, 25-48)
Request bulk or custom format
Ask our AI about this Product

The Collibri PCR-free ES DNA Library Preparation Kit is designed for fast and convenient library preparation from inputs as low as 100 ng intact genomic DNA or already prepared amplicons (>300 bp) of any species. The entire library preparation protocol takes about two hours for the PCR-free workflow. If needed, Collibri Library Amplification Master Mix with primers can be bought separately. Or see the Collibri ES DNA Library Preparation Kit, designed for fast and convenient library preparation with PCR from a wide range of inputs (1–500 ng).

The Collibri PCR-free ES DNA Library Preparation Kit is designed for fast and convenient library preparation from inputs as low as 100 ng intact genomic DNA or already prepared amplicons (>300 bp) of any species. The entire library preparation protocol takes about two hours for the PCR-free workflow. If needed, Collibri Library Amplification Master Mix with primers can be bought separately. Or see the Collibri ES DNA Library Preparation Kit, designed for fast and convenient library preparation with PCR from a wide range of inputs (1–500 ng).

The Collibri PCR-free ES DNA Library Preparation Kit:
• Includes all necessary components in a single kit* for quick use
• Is empowered on miniaturization liquid handlers to cut on costs
• Provides in-process visual feedback for easier handling
•Is suitable for conventional automation to increase throughput

* The kit consists of enzymatic modification, size selection/clean-up, and indexed adapter-containing modules, except for the Core kit, which enables Collibri combination with desired indexed adapters

The Collibri PCR-free ES DNA Library Preparation Kit contains inert dyes that provide visual feedback of preparation progress: the reaction mixes change color in response to the addition of critical components at each step. Moreover, the enzymatic shearing (ES) module of the kit generates a consistently high yield of library, which is enough for library quantification  and sequencing and multiple runs on Illumina instruments.

Applications
The Collibri PCR-free ES DNA Library Preparation Kit is recommended for:
Whole genome sequencing
• Resequencing
• De novo assembly

For Research Use Only. Not for use in diagnostic procedures.
Specifications
DescriptionThe product contains modules A38575024, A43348024 , A43190024
For Use With (Application)Whole Genome Sequencing, Resequencing, De Novo Assembly
For Use With (Equipment)HiSeq X, HiSeq2500, HiSeq3000, HiSeq4000, MiSeq or iSeq, NextSeq 550, NextSeq 1000 & 2000, NovaSeq 6000
LibrariesFragment Library
No. of Reactions24 Preps
Product LineCollibri™
Product TypePCR-free ES DNA Library Prep Kit with UD Indexes (Set B, 25-48)
Quantity24 preps
Sample TypeDNA
Shipping ConditionDry Ice
SpeciesAll
Workflow StepLibrary Generation
FormatKit
Sequencing TypeGenome and DNA Sequencing
Unit SizeEach
Contents & Storage
• Collibri PCR-free ES DNA Library Prep Kit, store at -20°C
• Collibri DNA Library Cleanup Kit, store at 4°C
• Collibri DNA UD Indexes, store at -20°C
Have questions about this product? Ask our AI assisted search.
This is an AI-powered search and may not always get things right. You can help us make it better with a thumbs up or down on individual answers or by selecting the “Give feedback" button. Your search history and customer login information may be retained by Thermo Fisher and processed in accordance with our Privacy Notice.

Frequently asked questions (FAQs)

Can indexed adaptors or other modules be purchased separately for the Collibri DNA Library Prep kits?

Indexed adapters and other modules in the kit can be purchased separately. Please refer to thermofisher.com/collibriDNA and contact us for more details.

With the Collibri DNA Library Prep kits, what is the distribution of enzymatically sheared DNA in the library?

Fragment size ranges from 150 bp to 850 bp. For optimal results, we recommend and provide detailed instructions for 350 bp and 550 bp average size library construction.

With the Collibri DNA Library Prep kits, can I use indexed adaptors interchangeably between RNA-seq and DNA-seq applications?

No. They are specifically designed for a product and cannot be used interchangeably.

With the Collibri DNA Library Prep kits, why is my ligation master mix orange instead of red?

Dyes inside the master mix react with CO2 and change the color from red to orange. The mix is still usable when the color changes to orange. To prevent discoloration, avoid keeping it on dry ice.

Are Collibri DNA Library Prep kits compatible with FFPE samples?

Collibri PS DNA Library Prep Kit can be used for library construction from lower-quality DNA (such as FFPE samples). However, the yields of adaptor-ligated molecules are typically lower compared to the yields obtained with high-quality DNA.

Collibri ES DNA Library Prep Kit is not recommended for library construction from FFPE samples.

Documents & Downloads

Certificates

Safety Data Sheets

Share catalog number, name or link