This fluorescence-based Electrophoretic Mobility Shift Assay (EMSA) Kit provides a fast, easy and quantitative method to detect both nucleic acid and protein in the same gel. This kit uses two fluorescent dyes for detection - SYBR™ Green EMSA nucleic acid gel stain for RNA or DNA detection and SYPRO™ Ruby EMSA protein gel stain for protein detection. The nucleic acids and proteins are stained sequentially in the gel after electrophoresis, so there is no possibility that labeling will interfere with the protein binding being assayed. Staining only takes about 20 minutes for nucleic acid, and then about 4 hours for the subsequent protein staining.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Assay
EMSA (Electrophoretic Mobility Shift Assay)
Detection Method
Fluorescent
Product Type
Electrophoretic Mobility-Shift Assay (EMSA) Kit
Product Line
SYBR™, SYPRO™
Quantity
1 kit
Shipping Condition
Room Temperature
Contents & Storage
Store in freezer (-5 to -30°C) and protect from light.