Phusion High-Fidelity PCR Master Mix with HF or GC Buffer
Phusion High-Fidelity PCR Master Mix with HF or GC Buffer
Thermo Scientific™

Phusion High-Fidelity PCR Master Mix with HF or GC Buffer

Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.
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Catalog NumberIncludesNo. of Reactions
F532SGC Buffer100 Reactions
F532LGC Buffer500 Reactions
F531LHF Buffer500 Reactions
F531SHF Buffer100 Reactions
4 Options
Catalog number F532S
Price (JPY)/ 100 reactions
Price:37,300
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Includes:
GC Buffer
No. of Reactions:
100 Reactions
Request bulk or custom format
Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR. Featuring an error rate 50-fold lower than that of Taq and 6-fold lower than that of Pfu, Phusion High-Fidelity DNA Polymerase is excellent choice for cloning and other applications requiring high fidelity. Phusion DNA polymerases offer robust performance with short protocol times, even in the presence of PCR inhibitors, and generate higher yields with lower enzyme amounts than other DNA polymerase.

Phusion High-Fidelity PCR Master Mix is convenient 2X mix containing Phusion DNA Polymerase, nucleotides, and optimized reaction buffer including MgCl2. Two master mix formulations are available: with HF Buffer (F-531S and F-531L) and with GC Buffer (F-532S and F-532L). The error rate of Phusion DNA Polymerase in HF Buffer (4.4 x 10-7) is lower than that in GC Buffer (9.5 x 10-7). Therefore, the master mix with HF Buffer should be used as the default master mix for high-fidelity amplification. However, GC Buffer can improve the performance of Phusion DNA Polymerase on some difficult or long templates, such as GC-rich templates or those with complex secondary structures.

Highlights

  • High fidelity (25X Taq)
  • Fast PCR due to short extension times (15–30 s/kb)
  • Robust performance, minimal optimization needed

Applications

  • Amplification of difficult (GC-rich) templates
  • High-fidelity PCR
  • Cloning
  • Template generation for sequencing
  • Long-range PCR (up to 20 kb)
  • Mutagenesis
  • High throughput PCR

Using Phusion DNA polymerases

Annealing rules for Phusion DNA polymerases are different from many common DNA polymerases (such as Taq DNA polymerases).

For Research Use Only. Not for use in diagnostic procedures.
Specifications
GC-Rich PCR PerformanceHigh
PolymerasePhusion High-Fidelity DNA Polymerase
Reaction SpeedFast
Product TypeHigh-Fidelity PCR Master Mix
Shipping ConditionDry Ice
For Use With (Application)Standard PCR, High-fidelity PCR
Concentration2X
Fidelity (vs. Taq)52X
Hot StartNo
No. of Reactions100 Reactions
OverhangBlunt
Reaction FormatSuperMix or Master Mix
Size (Final Product)20 kb or less
IncludesGC Buffer
Unit Size100 reactions
Contents & Storage
• 2X Phusion Master Mix with GC Buffer (2 x 1.25 mL)
• 100% DMSO (1 x 500 μL)

Store at –20°C.