Bovine Genotypes Panel 1.2 Kit

Thermo Scientific Bovine Genotypes Panel 1.2 kit is offered for cattle parentage testing and identification using Short Tandem Repeat (STR) DNA loci.

The Bovine Genotypes Panel 1.2 allows co-amplification of the 12 microsatellites in a single multiplex PCR reaction. One primer from each primer pair is end-labeled with a fluorescent dye. After PCR, the fragments are separated and detected in a single electrophoresis injection, using an automated electrophoresis instrument (see Figure 1). In addition, the kit includes bovine control DNA for verification of acceptable PCR and electrophoresis conditions. Reagents and protocols of the Bovine Genotypes Panel 1.2 have been optimized to deliver similar peak sizes (amplification yields) for each loci. Allele callings obtained with this kit represent the true alleles of an individual, instead of 'plus-A peaks' or 'split peaks' typically interpreted when using e.g. a conventional Taq DNA polymerase. The kit employs Phusion Hot Start DNA Polymerase that has a proofreading activity and eliminates the 'plus-A peaks'.

Thermo Scientific Bovine Genotypes Panel 1.2 encompasses all the 12 STR loci recommended by the International Society for Animal Genetics (ISAG) for routine use in bovine parentage testing and identification, including: TGLA227, BM2113, TGLA53, ETH10, SPS115, TGLA126, TGLA122, INRA23, BM1818, ETH3, ETH225, and BM1824. The primer sequences employed by the kit are identical to those recommended by the Cattle Molecular Markers and Parentage Testing standing committee of ISAG, and they have been validated in extensive laboratory comparison tests.

Highlights

• Various kit choices that include STR loci recommended by ISAG
• Single multiplex reaction - less pipetting steps
• Optimized reagents
• Control DNA provided for verification of PCR and electrophoresis conditions
• Allele calling represents true alleles (no plus-A peaks)