PCR Master Mix (2X)
PCR Master Mix (2X)
Thermo Scientific™

PCR Master Mix (2X)

Thermo Scientific PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers.
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Catalog NumberNo. of Reactions
K01721000 Reactions
K0171200 Reactions
2 Options
Catalog number K0172
Price (JPY)/ 1,000 reactions
Price:67,300
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No. of Reactions:
1000 Reactions
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Thermo Scientific PCR Master Mix is a 2X concentrated solution of Taq DNA Polymerase, dNTPs, and all of the components required for PCR, except DNA template and primers. This pre-mixed formulation saves time and reduces contamination due to a reduced number of pipetting steps required for PCR set up. The mix is optimized for efficient and reproducible PCR.

Highlights

  • Convenient, ready-to-use mix
  • Thermostable—half life is more than 40 min. at 95°C
  • Generates PCR products with 3'-dA overhangs
  • Incorporates modified nucleotides (e.g., biotin-, digoxigenin-, fluorescently-labeled nucleotides)

Applications

  • Routine PCR amplification of DNA fragments up to 5 kb
  • High throughput PCR
  • DNA labeling

Note

The error rate of Taq DNA Polymerase in PCR is 2.2 x 10-5 errors per nt per cycle, as determined by a modified method described in. Accordingly, the accuracy of PCR is 4.5 x 104. Accuracy is an inverse of the error rate and shows an average number of correct nucleotides incorporated before an error occurs.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
GC-Rich PCR PerformanceLow
PolymeraseTaq DNA Polymerase
Reaction SpeedStandard
Product TypePCR Master Mix
Shipping ConditionDry Ice
For Use With (Application)Standard PCR
Concentration2X
Fidelity (vs. Taq)1X
Hot StartNo
No. of Reactions1000 Reactions
Overhang3'-A
Reaction FormatSuperMix or Master Mix
Starting MaterialDNA
Unit Size1,000 reactions
Contents & Storage
• 2X PCR Master Mix, 20 x 1.25 mL
• Nuclease-free water, 20 x 1.25 mL

Store at -20°C.