Search Thermo Fisher Scientific
- Contact Us
- Quick Order
-
Don't have an account ? Create Account
Invitrogen™
PureLink™ PCR Purification Kit
The PureLink PCR Purification Kit provides a more convenient, rapid, and high-yielding purification process compared to other companies' kits.
Change view
| Catalog Number | Quantity |
|---|---|
| K310001 | 50 Preps |
| K310002 | 250 Preps |
Catalog number K310001
Price (JPY)Contact Us ›
19,400
Each
Quantity:
50 Preps
The PureLink PCR Purification Kit provides a more convenient, rapid, and high-yielding purification process compared to other companies' kits. After PCR amplification, purification of the PCR amplicons is required for many common downstream applications, including enzyme digestion, ligation, sequencing, and labeling experiments, to prevent enzymes (polymerase, restriction enzyme) and reaction components (dNTPs, primers, buffers, salts) from carrying over, contaminating, and negatively influencing the downstream experiments.
Advantages of the PureLink PCR Purification Kit include:
- 4X binding capacity compared to Company Q; 40 μg for the PureLink kit versus 10 μg from other companies' kits
- No need for tedious sample pH adjustments necessary with other kits
- Convenient and rapid protocol, completed in less than 10 minutes
- Optional size-selection buffers included for selectively purifying 300–600 bp fragments
The PureLink PCR Purification Kit is based on the selective binding of dsDNA to silica-based membrane in the presence of chaotropic salts. Simply mix the PCR product with Binding Buffer and apply to the PureLink Spin Column. The dsDNA binds to the silica-based membrane in the column. Remove impurities by thorough washing with Wash Buffer. To purify the DNA, elute the dsDNA in low-salt Elution Buffer or water.The purified PCR product is suitable for automated fluorescent DNA sequencing, restriction enzyme digestion, and cloning.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
FormatKit
High-throughput CompatibilityHigh-throughput Compatible
Isolation TechnologySilica Spin Column
Product LinePureLink™
Product TypePCR Purification Kit
Quantity50 Preps
Run Time10 min.
Sample TypePCR Products
Shipping ConditionRoom Temperature
TargetDNA from Enzymatic Reactions (e.g. PCR)
For Use With (Application)Next-Generation Sequencing (NGS), Cloning
Unit SizeEach
Contents & Storage
For better long-term performance, it is recommended to store the purification columns at 2°C to 8°C.
Have questions about this product? Ask our AI assisted search.
This is an AI-powered search and may not always get things right. You can help us make it better with a thumbs up or down on individual answers or by selecting the “Give feedback" button. Your search history and customer login information may be retained by Thermo Fisher and processed in accordance with our
Privacy Notice.