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Invitrogen™
pcDNA™3.1/V5-His TOPO™ TA Expression Kit
The pcDNA™3.1/V5-His TOPO™ TA Expression Kit offers one-step cloning of Taq-amplified PCR products into a high-level expression vector. Topoisomerase activation Read more
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| Catalog Number | Quantity | Vector |
|---|---|---|
| K480040 | 40 Reactions | TOPO-TA Vectors, pcDNA |
| K480001 | 20 Reactions | TOPO-TA Vectors, pcDNA |
Catalog number K480040
Price (JPY)/ 40 reactionsContact Us ›
Price:233,000
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Quantity:
40 Reactions
Vector:
TOPO-TA Vectors, pcDNA
The pcDNA™3.1/V5-His TOPO™ TA Expression Kit offers one-step cloning of Taq-amplified PCR products into a high-level expression vector. Topoisomerase activation of the pcDNA3.1/V5-His-TOPO™ vector allows PCR products to be ligated in just 5 minutes on your bench top and results in 90% recombinants.
In addition, the vector includes the following features:
• Strong CMV promoter for high-level, constitutive expression.
• C-terminal V5 epitope tag for efficient detection of recombinant proteins with an Anti-V5 antibody.
• C-terminal polyhistidine (6xHis) sequence for purification using nickel-chelating resin and detection with an Anti-His (C-term) antibody.
In addition, the vector includes the following features:
• Strong CMV promoter for high-level, constitutive expression.
• C-terminal V5 epitope tag for efficient detection of recombinant proteins with an Anti-V5 antibody.
• C-terminal polyhistidine (6xHis) sequence for purification using nickel-chelating resin and detection with an Anti-His (C-term) antibody.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Constitutive or Inducible SystemConstitutive
Delivery TypeTransfection
PromoterCMV
Product TypeTOPO TA Expression Kit
Selection Agent (Eukaryotic)Geneticin™ (G-418)
Protein TagHis Tag (6x), V5 Epitope Tag
Cloning MethodTOPO-TA
Quantity40 Reactions
VectorTOPO-TA Vectors, pcDNA
Product LineTOPO™, pcDNA™
For Use With (Application)Constitutive Expression
Unit Size40 reactions
Contents & Storage
2 × Box 1 (store at -20°C)
• 20 μl pcDNA™3.1/V5-His-TOPO™ (10 ng/μl)
• 100 μl 10X PCR Buffer
• 10 μl dNTP Mix (50 mM)
• 50 μl Salt Solution
• 1 ml Sterile Water
• 10 μl Control PCR Template (50 ng/μl)
• 10 μl Control PCR Primers (100 ng/μl each)
• 20 μl T7 Sequencing Primer (100 ng/μl)
• 20 μl BGH Reverse Sequencing Primer (100 ng/μl)
• 10 μl Expression Control Plasmid (500 ng/μl)
2 × Box 2 (store at -80°C)
• 21 × 50 μl TOP10 E. coli cells
• 50 μl pUC19 Control DNA (10 pg/μl)
• 6 ml S.O.C. Medium
• 20 μl pcDNA™3.1/V5-His-TOPO™ (10 ng/μl)
• 100 μl 10X PCR Buffer
• 10 μl dNTP Mix (50 mM)
• 50 μl Salt Solution
• 1 ml Sterile Water
• 10 μl Control PCR Template (50 ng/μl)
• 10 μl Control PCR Primers (100 ng/μl each)
• 20 μl T7 Sequencing Primer (100 ng/μl)
• 20 μl BGH Reverse Sequencing Primer (100 ng/μl)
• 10 μl Expression Control Plasmid (500 ng/μl)
2 × Box 2 (store at -80°C)
• 21 × 50 μl TOP10 E. coli cells
• 50 μl pUC19 Control DNA (10 pg/μl)
• 6 ml S.O.C. Medium