The SilverQuest Silver Staining Kit provides a rapid and easy method to silver stain proteins and DNA in polyacrylamide gels. It is specifically designed to provide sensitive silver staining compatible with mass spectrometry analysis.
Features of this kit: • Compatible—staining compatible with mass spectrometry analysis • Protocol—protein bands (0.3 ng of protein) can be visualized within an hour or for added speed a microwave procedure can be completed in ∼30 minutes • Color-coded solutions—color-coded solutions for easy staining protocol
Mass spectrometry-compatible reagents The SilverQuest Silver Staining Kit has been specifically designed to make protein detection compatible with mass spectrometry analysis. The SilverQuest reagent set contains a sensitizer that contains no glutaraldehyde or formaldehyde, so it leaves lysine and arginine side-chains unmodified. Additionally, the SilverQuest destaining solution effectively removes silver from protein bands in polyacrylamide gels. Peptides remain as efficient targets for in-gel trypsin digestion and gel extraction and yield better coverage during mass spectrometry analysis.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Detection Location
In-Gel Detection
Detection Method
Colorimetric
Label or Dye
SilverQuest Silver Stain
Product Type
Protein Gel Staining Kit
Shelf Life
6 Months
Product Line
SilverQuest™
Quantity
1 kit
Target Molecule
Protein
Contents & Storage
This kit contains sufficient reagents to stain 25 minigels
Contains: 250 mL Sensitizer 25 mL Stainer 250 mL Developer 2 mL Developer Enhancer 250 mL Stopper solution 60 mL Destainer A 60 mL Destainer B
Store kit at room temperature (15°C to 30°C). Stable for 6 months.