NucBlue™ Fixed Cell ReadyProbes™ Reagent (DAPI)
NucBlue™ Fixed Cell ReadyProbes™ Reagent (DAPI)
Invitrogen™

NucBlue™ Fixed Cell ReadyProbes™ Reagent (DAPI)

DAPI is a commonly used nuclear counterstain for fixed cells that emits blue fluorescence when bound to DNA. With NucBlueRead more
Catalog NumberQuantity
R376066 vial(s) kit
Catalog number R37606
Price (JPY)
20,300
Each
Add to cart
Quantity:
6 vial(s) kit
Price (JPY)
20,300
Each
Add to cart
Ask our AI about this Product
DAPI is a commonly used nuclear counterstain for fixed cells that emits blue fluorescence when bound to DNA. With NucBlue Fixed Cell ReadyProbes Reagent, we have formulated a high-purity form of this classic stain in a room temperature-stable solution that is provided in a convenient-to-use dropper bottle. Just tip and drip two drops per ml to stain your cells.

Also available: DAPI (4',6-diamidino-2-phenylindole, dihydrochloride) at 12.5X concentration (5 μM) in water.

• No need to dilute, weigh, or pipette
• Convenient dropper bottle—just use two drops per mL
• Stable at room temperature—keep handy at your scope or cell culture area• DAPI is excited with UV light and detected through a blue/cyan filter

See other ReadyProbes reagents for cell staining
See other nuclear stains for imaging

Cell imaging applications
DAPI is a classic fluorescent dye used extensively for nuclear staining of fixed cells. In fluorescence microscopy, DAPI is excited with UV light and detected through a blue/cyan filter (Figure 1)..

Suggestions for use
In most cases 2 drops/ml of NucBlue Fixed Cell Stain and an incubation of 15 to 30 minutes will produce bright nuclear staining; however, optimization may be needed for some cell types, conditions, and applications. In such cases, simply add more, or fewer, drops until the optimal staining intensity is obtained.
• NucBlue Fixed Cell Stain is excited by UV light at 360 nm when bound to DNA, with an emission maximum at 460 nm. It is detected through a blue/cyan filter, such as a DAPI filter, blue GFP filters, or the Semrock BrightLine Alexa Fluor 350 Dye filter set.
• As a preferred blue nuclear stain in fixed cell imaging experiments, NucBlue Fixed Cell Stain is ideal for use with antibody-based applications.

Visualize staining your cell without wasting your reagents, antibodies, or time with our new Stain-iT Cell Staining Simulator.

For Research Use Only. Not for use in diagnostic procedures.
Specifications
ColorBlue
Detection MethodFluorescence
Dye TypeDAPI
EmissionVisible
Excitation Wavelength Range360⁄460
For Use With (Equipment)Fluorescence Microscope, Flow Cytometer
FormLiquid
Product LineMolecular Probes™
Quantity6 vial(s) kit
Shipping ConditionRoom Temperature
Label TypeFluorescent Dye
Product TypeNucleic Acid Stain
SubCellular LocalizationNucleus
Unit SizeEach
Contents & Storage
6 × 2.5 mL dropper bottles

Store at ≤ 25°C

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Frequently asked questions (FAQs)

My DAPI labeled samples have a strong blue background signal immediately after mounting. What can I do to fix this?

Some mounting medias can have strong blue autofluorescence. If you are seeing a high blue background, it could be coming from the mountant. Try labeling the sample and view it before (using a wet mount in buffer) and after mounting to determine if the background signal is coming from the mounting media or the sample itself.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Do you offer an alternative for the discontinued 3-Germ Layer ICC Kit (Cat. No. A25538)?

We do not have a direct alternative for the discontinued 3-Germ Layer ICC Kit (Cat. No. A25538). However, we do have alternative primary and secondary antibodies, as well as reagents, that can be used for trilineage differentiation. Please note that we have not internally validated the use of all these reagents together.

We recommend the following primary antibodies:

Alpha-Smooth Muscle Actin Monoclonal Antibody (1A4 (asm-1)) (Cat. No. MA5-11547)

alpha-Fetoprotein Monoclonal Antibody (AFP3) (Cat. No. 14-6583-80)

beta-3 Tubulin Monoclonal Antibody (2G10) (Cat. No. MA1-118)

We recommend the following secondary antibodies:

Goat anti-Mouse IgG2a Cross-Adsorbed, Alexa Fluor 555 (Cat. No. A21137)

Goat anti-Mouse IgG2a Cross-Adsorbed, Alexa Fluor 594 (Cat. No. A21135)

https://www.thermofisher.com/antibody/product/Goat-anti-Mouse-IgG1-Cross-Adsorbed-Secondary-Antibody-Polyclonal/A-21121

We recommend using the following reagents:

https://www.thermofisher.com/order/catalog/product/88-8824-00

NucBlue Fixed Cell ReadyProbes Reagent (DAPI) (Cat. No. R37606)

Blocker BSA (Cat. No. 37520)

DPBS (10X), no calcium, no magnesium (Cat. No. 14200075)

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

Can I use the ReadyProbes reagents for flow cytometry?

This is not recommended. The ReadyProbes reagents were developed for imaging applications whereas the Ready Flow reagents were optimized for flow cytometry.

Find additional tips, troubleshooting help, and resources within our Cell Analysis Support Center.

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