pcDNA5⁄FRT vector is a 5.1 kb expression vector designed for use with the Flp-In™ System. This vector features: 1. CMV promoter for high level expression in mammalian cells; 2. ten unique restriction site for easy cloning; 3. FLP Recombination Target (FRT) site for Flp recombinase-mediated integration of the vector into the Flp-In™ host cell line and 4. Hygromycin resistance gene for selection of stable cell lines.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Constitutive or Inducible System
Constitutive
Delivery Type
Transfection
Promoter
CMV
Product Type
Mammalian Expression Vector
Selection Agent (Eukaryotic)
Hygromycin
Protein Tag
Untagged
Cloning Method
Restriction Enzyme/MCS
Quantity
20 μg
Vector
pcDNA
Product Line
Flp-In™, pcDNA™
For Use With (Application)
Targeted Integration
Unit Size
20 µg
Contents & Storage
Contains both pcDNA5⁄FRT and pcDNA5⁄FRT⁄CAT vectors. Store at -20°C