Optimized for researchers performing RNA structure, RNA sequencing, protein footprinting, and boundary experiments, Ambion™ RNase T1, RNA-Grade cleaves 3' of single-stranded G residues and is tested for purity to ensure that no contaminating ribonuclease activities are present that could cleave at unanticipated sites.200 µL(1 U/µL)入りのチューブ1本で提供。RNase T1 can be used to perform boundary experiments to define the minimal RNA sequence required for selectable activities such as protein binding or catalysis.In addition to applications for RNA structural analysis, RNA-Grade ribonucleases can be used to map protein binding sites on RNAs by comparing cleavage patterns in the presence and absence of an RNA binding protein.
Unit Definitions: 100 units is the amount of enzyme that yields an increase in absorption at 260 nm of 0.01428 units per min at room temperature using 60 µg/mL yeast total RNA as a substrate.
Accessory Products: Other RNA-Grade ribonuclease available include RNase A (SKU# AM2274), which cleaves 3' of single-stranded C and U residues.Combinations of the single- and double-stranded specific ribonucleases can provide rapid analysis of the physical structure of an interesting RNA.
研究用途にのみご使用ください。診断目的には使用できません。
仕様
濃度1 U/μL
フォーマットチューブ
酵素RNase
フィデリティ(vs. Taq)10X
使用対象(アプリケーション)リアルタイムPCR(qPCR)
反応数200
製品ラインAmbion™
製品タイプRNase T1 PCRキット
数量200 μL
出荷条件ドライアイス
電子線源RNA
形状Solution
Unit Size200 µL
組成および保存条件
RNase T1, 10X RNA Structure Buffer, 1X Alkaline Hydrolysis Buffer, Yeast RNA, Precipitation⁄Inactivation Buffer, and Gel Loading Buffer II should all be stored at –20°C.