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Application
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XRCC2 Antibody (MA5-58007) in WB
Western blot analysis of XRCC2 in A-549 and mouse heart (25 µg/lane). Samples were incubated overnight at 4°C with XRCC2 Recombinant Monoclonal Antibody (Product # MA5-58008) using a dilution of 1:1, 000. Followed by a HRP-conjugated Goat anti-Rabbit IgG (H+L) Secondary Antibody at 1:10,000 dilution. Blocking buffer: 3% nonfat dry milk in TBST; Exposure: 3 seconds.
Product Details
MA5-58007
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Expression System
HEK293 cells
Class
Recombinant Monoclonal
Type
Antibody
Clone
1T9D5
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-83 of human XRCC2 (NP_005422.1).
Conjugate
Unconjugated
Form
Liquid
Concentration
1 mg/mL
Purification
Protein A
Storage buffer
PBS, pH 7.3, with 0.05% BSA, 50% glycerol
Contains
0.09% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Dry ice (international)
RRID
AB_3681029
Product Specific Information
Immunogen sequence: MCSAFHRAES GTELLARLEG RSSLKEIEPN LFADEDSPVH GDILEFHGPE GTGKTEMLYH LTARCILPKS EGGLEVEVLF IDT
Target Information
The x-ray repair cross-complementing (XRCC) proteins are responsible for efficiently repairing and maintaining genetic stability following DNA base damage. These genes share sequence similarity with the yeast DNA repair protein Rad5. XRCC2 and XRCC3 are both involved in maintaining chromosome stability during cell division. XRCC2 is required for efficient repair of DNA double-strand breaks by homologous recombination between sister chromatids, and XRCC3 interacts directly with Rad51 to cooperate with Rad51 during recombinational repair.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: DKFZp781P0919; DNA repair protein XRCC2; recA/RAD51 family protein; X-ray repair complementing defective repair in Chinese hamster cells 2; X-ray repair cross complementing protein 2; X-ray repair cross-complementing protein 2
Gene Aliases: 4921524O04Rik; 8030409M04Rik; FANCU; RAD51; RecA; RGD1564823; XRCC2
UniProt ID: (Human) O43543, (Mouse) Q9CX47
Entrez Gene ID: (Human) 7516, (Mouse) 57434, (Rat) 499966
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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