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Invitrogen™
Taq DNA Polymerase (Brasil)
Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer. The enzyme consists of a single polypeptide with a molecular weight of 94 kDa.
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| Catalog Number | No. of Reactions |
|---|---|
| 11615010 | 500 Reactions |
| 11615036 | 100 Reactions |
| 11615025 | 1000 Reactions |
| 11615044 | 5000 Reactions |
Catalog number 11615010
Price (KRW)
-
No. of Reactions:
500 Reactions
Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer. The enzyme consists of a single polypeptide with a molecular weight of 94 kDa. It has a 5' to 3' DNA polymerase activity and a 5' to 3' exonuclease activity (see figure).
Taq DNA Polymerase native enzyme is purified from Thermus aquaticus YT1. Recombinant enzyme is purified from the cloned Thermus aquaticus DNA polymerase gene expressed in E. coli.
Features
- Choice of recombinant or native enzyme
- Amplification of PCR products up to 5 kb in size
- Enzyme is licensed and qualified for PCR
Applications
- Amplification of DNA from complex genomic, viral, and plasmid templates
- RT-PCR
- Sequencing ssDNA
- Cycle sequencing
Notes
- One unit of Taq DNA Polymerase is the amount of enzyme required to incorporate 10 nmol of deoxyribonucleotide into DNA in 30 min. at 74°C.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
GC-Rich PCR PerformanceLow
PolymeraseTaq DNA Polymerase
Reaction SpeedStandard
Quantity500 Units
Shipping ConditionDry Ice
For Use With (Application)RT-PCR
Concentration5 U/μL
Fidelity (vs. Taq)1X
Hot StartNo
No. of Reactions500 Reactions
Overhang3'-A
Size (Final Product)5 kb or less
Starting MaterialDNA
Unit SizeEach
Contents & Storage
• Taq DNA Polymerase (5 U/μL), 100 μL
• 10X PCR Buffer (without magnesium), 2.5 mL
• 50 mM MgCl2, 1 mL
Store at -20°C
• 10X PCR Buffer (without magnesium), 2.5 mL
• 50 mM MgCl2, 1 mL
Store at -20°C