Thermo Scientific Pierce SIA is a very short (1.5 angstrom) crosslinker for amine-to-sulfhydryl conjugation via N-hydroxysuccinimide (NHS) ester and iodoacetyl reactive groups.
Features of SIA:
• Reactive groups:NHS ester and iodoacetyl • Reactive toward: amino and sulfhydryl groups • Shortest sulfhydryl-reactive and amine-reactive heterobifunctional crosslinker available; compare to SIAB • NHS ester reacts with primary amines at pH 7-9 to form a stable amide bond • Iodoacetyl group reacts with sulfhydryl groups at pH > 7.5 to form stable thioether bond • Non-cleavable • Water-insoluble (dissolve first in DMF or DMSO)
Product References:
Crosslinker Application Guide -- search for recent literature references for this product
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Chemical Reactivity
Amine-Sulfhydryl
Cleavable
No
Description
SIA
Molecular Weight (g/mol)
283.02
PEGylated
No
Spacer Arm Length
1.5 Å
Cell Permeability
Yes
Shipping Condition
Ambient
Product Line
Pierce™
Labeling Method
Chemical Labeling
Crosslinker Type
Heterobifunctional
Reactive Moiety
Haloacetyl, NHS Ester
Spacer
Short (<10 Å)
Form
Solid
Quantity
50 mg
Solubility
DMF, DMSO
Format
Standard
Water Soluble
No
Product Type
Crosslinker
Contents & Storage
Upon receipt store at 4°C protected from moisture.