The Thermo Scientific™ Pierce™ High Capacity Streptavidin Chromatography Cartridges are convenient, ready-to-use devices for separating biotinylated molecules from non-biotinylated molecules and for purifying antigens using biotinylated antibodies. The chromatography cartridges contain a beaded resin of immobilized recombinant streptavidin protein that binds biotinylated proteins at greater than 10 mg/mL of beads. This corresponds to 2 to 3 times higher biotin-binding capacity than products from other suppliers and is ideal when a high amount of recovery is required.

These cartridges enable fast, easy, and reproducible chromatographic separations and are compatible with the major automated liquid-chromatography systems or manual syringe processing (see Table 1 for cartridge general properties). The cartridges attach directly to ÄKTA™ or FPLC systems without additional connectors. The included accessory pack readily adapts cartridges for use with Luer-Lok™ syringe fittings or 1/16' tubing. These cartridges can be regenerated multiple times for affinity purification.

Features of High Capacity Streptavidin Agarose:
• Streptavidin—purified recombinant streptavidin protein (53 kDa, near-neutral pI); tetrameric with four biotin-binding site per molecule
• Agarose resin—support is cross-linked 6% beaded agarose (CL-6B), the most popular resin for protein affinity purification methods
• Inert and stable—streptavidin is immobilized by charge-free, leach-resistant covalent bonds, resulting in low nonspecific binding and enabling multiple uses without decline in yield
• High capacity—this variety of beads has a dense load of immobilized streptavidin, providing a binding capacity greater than 10 mg of biotinylated BSA per mL of resin
• Two formats—available in several bottle sizes of resin slurry and as packed 1 mL and 5 mL chromatography cartridges

Properties of cross-linked 6% beaded agarose (CL-6B):
• Support pH stability: 2 to 14 (short term); 3 to 13 (long term)
• Average particle size: 45 to 165 microns
• Exclusion limit: 10,000 to 4,000,000 daltons
• Maximum recommended flow rate: 4 mL/minute (1 mL column), 5 mL/min (5 mL column)
• Maximum linear velocity: 30 cm per hour
• Maximum pressure: 0.3 MPa, 43.5 psi or 3 bar

Pierce High Capacity Streptavidin Chromatography Cartridges consist of purified recombinant streptavidin covalently immobilized at a high density onto high-quality cross-linked 6% beaded agarose. Biotinylated antibodies, proteins, peptides, nucleic acids, and other molecules or interaction complexes can be captured, immunoprecipitated, removed, or purified from samples using this streptavidin resin.

Streptavidin is a tetrameric protein containing four biotin-binding sites similar to avidin. The native protein from Streptomyces avidinii is carbohydrate-free, has an acidic pI of 5.5 and a mass of 75 kDa. The recombinant protein used to manufacture Pierce streptavidin resins has a near-neutral isoelectric point and a mass of 53 kDa. Streptavidin generally has less nonspecific binding than avidin (from chicken egg white) because of the absence of carbohydrates.

For more information see: Chromatography Cartridges for Desalting and Affinity Purification