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Catalog Number | No. of Reactions | Gel Compatibility |
---|---|---|
A44449 | A standard kit will label 40 mini gels or 40 mini gel-sized membranes | Any Gel Type and Gel Chemistry |
A44717 | A standard kit will label 10 mini gels or 10 mini gel-sized membranes | Mini Precast Protein Gels, Midi Precast Protein Gels |
Instant visualization of proteins in gels
Coomassie and other gel staining and de-staining steps can be extremely time consuming and cumbersome. No-Stain Protein Labeling Reagent forms covalent bonds with the lysine amino acid side chains on all proteins in a gel within 10 minutes. Since lysine is one of the most abundant amino acids, No-Stain reagent enables detection of all proteins in a gel or on a membrane, and the strong signal emission from the covalently bonded reagent provides nanogram-level sensitivity.
Achieve the gold standard for quantitative western blotting
Protein normalization is a critical step in obtaining reliable and reproducible quantitative western blotting. Total protein normalization is considered the gold standard for quantitative western blotting. Many leading journals have developed guidelines for submitting western blotting research and select quotes from those guidelines are provided below.
An accurate loading control should display a linear relationship between signal intensity and sample load in all experimental conditions. The signal intensity obtained from labeling of total proteins on a membrane with No-Stain reagent ensures a linear relationship between signal intensity and sample load (see figure below) in all experimental conditions. Therefore, the use of No-Stain reagent in quantitative western blot applications enables the use of total protein as an ideal loading control.
The shelf life of the No-Stain Protein Labeling Reagent is at least 2 years from the date of receipt of the product.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
Even though the No-Stain Protein Labeling Reagent (Cat. No. A44717) is light sensitive, membranes can be kept wet in the dark for at least 3 days with minimal effect on the signal. So, yes, it is possible.
Find additional tips, troubleshooting help, and resources within our Protein Gel Electrophoresis Chambers, Power Supplies, and Accessories Support Center.
Yes, the No-Stain Protein Labeling Reagent provides uniform labeling of proteins in gels or on membranes, provided they are imaged the same way.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
Yes, reproducible signal intensity will be obtained when using the No-Stain Protein Labeling Reagent to label either the same type, identically loaded gels or the same type membranes from the transfer of identically loaded, same-type gels: quantitation results will be similar between the gel pairs and the membrane pairs.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
The iBright imager now has software enabling Total Protein Normalization when using the No-Stain Protein Labeling Reagent. Additional data analysis software for the iBright imager is not being planned at this time.
Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.
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