No-Stain™ Protein Labeling Reagent
No-Stain™ Protein Labeling Reagent
Invitrogen™

No-Stain™ Protein Labeling Reagent

Green features
A fast, easy to use, covalent protein labeling reagent, applied to a membrane after gel transfer that provides sensitive, linear detection of protein for total protein normalization of western blotting data. The Invitrogen No-Stain Reagent can also be used as a fast, sensitive gel stain for visualization of proteins in a gel.
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Catalog NumberNo. of ReactionsGel Compatibility
A44449A standard kit will label 40 mini gels or 40 mini gel-sized membranesAny Gel Type and Gel Chemistry
A44717A standard kit will label 10 mini gels or 10 mini gel-sized membranesMini Precast Protein Gels, Midi Precast Protein Gels
Catalog number A44449
Price (KRW)
275,000
Each
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Add to cart
No. of Reactions:
A standard kit will label 40 mini gels or 40 mini gel-sized membranes
Gel Compatibility:
Any Gel Type and Gel Chemistry
Recurring order eligible. Learn more »
Price (KRW)
275,000
Each
Add to cart
Ask our AI about this Product
The Invitrogen No-Stain Protein Labeling Reagent provides a flexible, accurate, rapid, and reliable method to visualize and normalize proteins in a gel or on a membrane (post-transfer). It forms covalent bonds to proteins in gels or on membranes within 10 minutes, does not require any de-staining steps, and can be instantly visualized using any commonly available imager. No-Stain reagent does not require any particular gels or other reagents and is compatible with gel stains and western workflows.

Instant visualization of proteins in gels

Coomassie and other gel staining and de-staining steps can be extremely time consuming and cumbersome. No-Stain Protein Labeling Reagent forms covalent bonds with the lysine amino acid side chains on all proteins in a gel within 10 minutes. Since lysine is one of the most abundant amino acids, No-Stain reagent enables detection of all proteins in a gel or on a membrane, and the strong signal emission from the covalently bonded reagent provides nanogram-level sensitivity.

  • Alternative to traditional gel staining reagents—provides more accurate normalization over a wide range of protein lysate concentrations (1–80 μg)
  • Sensitive—lower limit of detection of 20 ng per band
  • Specific—forms bonds only with the lysine side chains of proteins. Unbound reagent does not emit, thereby enabling a superior signal-to-noise ratio
  • Flexible—no need to change your gels to get stain-free convenience. No-Stain reagent provides stain-free convenience with any gel type (precast or pour-your-own gel)

Achieve the gold standard for quantitative western blotting

Protein normalization is a critical step in obtaining reliable and reproducible quantitative western blotting. Total protein normalization is considered the gold standard for quantitative western blotting. Many leading journals have developed guidelines for submitting western blotting research and select quotes from those guidelines are provided below.

  • 'For quantitative comparisons, appropriate reagents, controls and imaging methods with linear signal ranges should be used' – Nature
  • 'Record how data were obtained, whether signal intensity was linear with antigen loading, and how protein loading was normalized' – Journal of Biological Chemistry
  • 'Normalize signal intensity to total protein loading (assessed by staining membranes for total protein) whenever possible' – Journal of Biological Chemistry
  • 'House-keeping proteins should not be used for normalization without evidence that manipulations do not affect expression' – Journal of Biological Chemistry

An accurate loading control should display a linear relationship between signal intensity and sample load in all experimental conditions. The signal intensity obtained from labeling of total proteins on a membrane with No-Stain reagent ensures a linear relationship between signal intensity and sample load (see figure below) in all experimental conditions. Therefore, the use of No-Stain reagent in quantitative western blot applications enables the use of total protein as an ideal loading control.

  • Easy-to-use protocol—labeling of proteins within 10 minutes on either nitrocellulose or PVDF membranes
  • Rapid visualization using a wide-range of imagers with UV or fluorescence light sources
  • Accurate total protein normalization—the broad linear range for protein detection of 1–80 μg enables detection of No-Stain signal along with that of your target protein to achieve accurate total protein normalization
  • Sensitive and stable—nanogram level sensitivity with a stable signal that is compatible with downstream immunodetection steps
  • Superior analysis—housekeeping proteins are susceptible to signal saturation and other biological variations which are not observed when using No-Stain reagent for total protein normalization

Learn more about No-Stain Protein Labeling Reagent ›

For Research Use Only. Not for use in diagnostic procedures.
Specifications
DescriptionThe No-Stain Protein Labeling Reagent forms covalent bonds with the lysine amino acid side chains on all proteins
For Use With (Application)Compatible with all downstream immunodetection steps (blocking, antibody binding, chemiluminescent or fluorescence based detection
For Use With (Equipment)Compatible with wide-range of imagers with UV or Fluorescence light sources, for example, the iBright imager
Green FeaturesLess hazardous
No. of ReactionsA standard kit will label 40 mini gels or 40 mini gel-sized membranes
Quantity40 reactions
Run Time10 minutes
Shelf LifeAt least 2 years from receipt
Shipping ConditionDry Ice
Detection MethodChemiluminescence, Fluorescence
Gel CompatibilityAny Gel Type and Gel Chemistry
Membrane CompatibilityNitrocellulose, PVDF
Product LineInvitrogen™
Product TypeProtein Labeling Reagent
Unit SizeEach
Contents & Storage
• No-Stain activator, 800 μL, store at -20°C
• No-Stain derivatizer, 800 μL, store at -20°C
• No-Stain labeling buffer (20X), 2 x 20 mL, store at 15°C to 30°C
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Certificates

Lot #Certificate TypeDateCatalog Number(s)
3148601Certificate of AnalysisMay 29, 2025A44449
3130363Certificate of AnalysisMay 09, 2025A44449
3124557Certificate of AnalysisApr 18, 2025A44717
3116036Certificate of AnalysisApr 08, 2025A44449
3108342Certificate of AnalysisMar 03, 2025A44449
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Safety Data Sheets

Frequently asked questions (FAQs)

The shelf life of the No-Stain Protein Labeling Reagent is at least 2 years from the date of receipt of the product.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Even though the No-Stain Protein Labeling Reagent (Cat. No. A44717) is light sensitive, membranes can be kept wet in the dark for at least 3 days with minimal effect on the signal. So, yes, it is possible.

Find additional tips, troubleshooting help, and resources within our Protein Gel Electrophoresis Chambers, Power Supplies, and Accessories Support Center.

Yes, the No-Stain Protein Labeling Reagent provides uniform labeling of proteins in gels or on membranes, provided they are imaged the same way.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

Yes, reproducible signal intensity will be obtained when using the No-Stain Protein Labeling Reagent to label either the same type, identically loaded gels or the same type membranes from the transfer of identically loaded, same-type gels: quantitation results will be similar between the gel pairs and the membrane pairs.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

The iBright imager now has software enabling Total Protein Normalization when using the No-Stain Protein Labeling Reagent. Additional data analysis software for the iBright imager is not being planned at this time.

Find additional tips, troubleshooting help, and resources within our Protein Assays and Analysis Support Center.

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