LguI (SapI) (5 U/μL)
LguI (SapI) (5 U/μL)
LguI (SapI) (5 U/μL)
LguI (SapI) (5 U/μL)
Thermo Scientific™

LguI (SapI) (5 U/μL)

5' G C T C T T C N1↓ 3'3' C G A G A A G N4↑ 5'Thermo ScientificRead more
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Catalog NumberQuantity
ER1931100 units
ER1932500 units
Catalog number ER1931
Price (KRW)
104,000
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Ends: 30-Sep-2025
122,000
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Quantity:
100 units
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Price (KRW)
104,000
Exklusiv online
Ends: 30-Sep-2025
122,000
Save 18,000 (15%)
Each
Add to cart
5'  G  C  T  C  T  T  C  N1 3'
3'  C  G  A  G  A  A  G  N4 5'

Thermo Scientific LguI (SapI) restriction enzyme recognizes GCTCTTC(1/4)^ sites and cuts best at 37°C in Tango buffer (isoschizomers: BspQI, PciSI, SapI). See Reaction Conditions for Restriction Enzymes for a table of enzyme activity, conditions for double digestion, and heat inactivation for this and other restriction enzymes. Note: Also available as a FastDigest enzyme for rapid DNA digestion.

Thermo Scientific conventional restriction endonucleases are a large collection of high quality restriction enzymes, optimized to work in one of the buffers of the Five Buffer System. In addition, the universal Tango buffer is provided for convenience in double digestions. All of the enzymes exhibit 100% activity in the recommended buffer and reaction conditions. To ensure consistent performance, Thermo Scientific restriction enzyme reaction buffers contain premixed BSA, which enhances the stability of many enzymes and binds contaminants that may be present in DNA preparations.

Features

• Superior quality—stringent quality control and industry leading manufacturing process
• Convenient color-coded Five Buffer System
• Includes universal Tango buffer for double-digestions
• BSA premixed in reaction buffers
• Wide selection of restriction endonuclease specificities

Applications

• Molecular cloning
• Restriction site mapping
• Genotyping
• Southern blotting
• Restriction fragment length polymorphism (RFLP)
• SNP

Note: For methylation sensitivity, refer to product specifications.
For Research Use Only. Not for use in diagnostic procedures.
Specifications
Compatible Buffer10X Buffer Tango
Product TypeRestriction Enzyme
Quantity100 units
Concentration5 U/μL
EnzymeLguI (SapI)
Methylation SensitivityNot Dam Methylation-Sensitive, Not Dcm Methylation-Sensitive, Not CpG Methylation-Sensitive
Optimal Reaction Temperature37°C
Research CategoryScarless Cloning
Sensitive to Heat InactivationYes
Type IIS REYes
Unit SizeEach

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