For high-resolution mapping and analysis of oligosaccharides released from glycoproteins, use the Thermo Scientific™ Dionex™ CarboPac™ PA200 Analytical & Guard Columns to provide sensitive and specific detection. Combined with pulsed amperometric detection (PAD), this anion-exchange column provide fast, reproducible separations with no need for derivatization. The hydrophobic, polymeric, pellicular anion-exchange resin packing material is stable over the range of pH 0–14. This unique pH-stability allows the use of eluent compositions that are conducive to anodic oxidation of carbohydrates at gold electrodes.
Fast, pH-Stable Separation of Oligosaccharides - Very high-efficiency separations
- Separations based on size, charge, degree of branching, and linkage isomerism
- Low flow rate saves on eluent consumption
About the Dionex CarboPac Family of Columns
The Dionex CarboPac family offers a selection of high-performance liquid chromatography (HPLC) columns, each optimized for a different class of compounds. Combined with PAD, which provides a means to detect carbohydrates without derivatization, these columns support simple, reliable techniques to separate sugar alcohols, mono- and disaccharides, and poly- and oligosaccharides.
Dionex CarboPac columns provide high-resolution separations of:
- Glycoprotein oligosaccharides and complex carbohydrates from dietary fiber, including fructans, maltodextrins, and amylopectins
- Monosaccharides, such as those obtained from glycoprotein hydrolysates or plant hydrolysates
- Mono- and disaccharides
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