Recombinant protein G is produced in a strain of E. coli which contains the gene for protein G from Streptococcus sp. This product is prepared by covalently coupling highly purified rec-Protein G to Sepharose™ 4B. It is supplied as a 50% suspension containing 25 ml rec-Protein G-Sepharose™ 4B + 25 ml PBS (10 mM phosphate-buffered saline, pH 7.4, containing 0.19% Kathon CG/ICP).

Applications: Immunoglobulin affinity purification and Immunoprecipitation.

Recombinant protein G binds to the Fc region of many types of immunoglobulin G including:

• all four subclasses of human IgG
• mouse IgG
• rabbit IgG
• guinea pig IgG
• rat IgG
• goat IgG
• sheep IgG
• cow IgG
• horse IgG

Recombinant protein G does not bind to IgG from chicken or cat, or to human IgA, IgM, or serum albumin. It binds weakly to IgG from dog.