Use this kit to isolate pure and viable untouched T-cells from PBMC by negative isolation. The kit depletes B cells, NK cells, monocytes, platelets, dendritic cells, granulocytes and erythrocytes. The negatively isolated human T-cells are left in the sample and have not been in contact with the Dynabeads. The bead and antibody-free T-cells are truly untouched, and in perfect shape for any functional assay and application.
• High purity, recovery and viability of human untouched T cells • Easy to use and to scale up • No columns required
Methodology: An antibody mix towards the non-T cells is added to the sample and allowed to bind to the cells. Dynabeads™ are added and will bind to the antibody-labeled cells during a short incubation. The bead-bound cells are quickly separated on a magnet and discarded. The remaining negatively isolated and untouched human T-cells can be directly analyzed in a flow cytometer and used in any application.
Note: A negative isolation strategy isolates pure and truly untouched cells. When maximum cell purity is critical, a positive cell isolation strategy is recommended
Starting Sample: PBMC
For Research Use Only. Not for use in diagnostic procedures.
사양
세포 유형
T Cells (Whole Population)
제품 유형
Cell Isolation Kit
최종 제품 유형
Cells
분리 기술
Negative Isolation
셀 수
Processes ∼1 x 109 cells total
테스트 수
100 Isolations
출력 생존력
>95%
제품라인
DYNAL™, Dynabeads™, Untouched™
수량
1 kit
샘플 종류
PBMC
배송 조건
Room Temperature
시작 물질 셀 수
1 x 107 PBMCs per isolation
타겟 종
Human
구성 및 보관
The Kit Contains: 10 mL Depletion Dynabeads™ and 2 mL Antibody Mix with monoclonal antibodies towards human CD14, CD16 (a and b), CD19, CD36, CD56, CD123 and CD235a (Glycophorin A).