The Baculovirus Expression System with Gateway™ Technology is designed to create recombinant pFastBac™ plasmids containing the polyhedrin promoter. The Baculovirus Expression System with Gateway™ Technology offers:
• Expression using the Bac-to-Bac™ technology (1) • Destination vectors carrying the polyhedrin promoter for production of native (pDEST™8), N-terminal histidine fusion (pDEST™10), and N-terminal GST fusion (pDEST™20) proteins • pDEST™10 vector containing a TEV protease cleavage site for removal of the fusion tag after protein purification (2)
For Research Use Only. Not for use in diagnostic procedures.
사양
프로모터
Polyhedrin
제품 유형
Expression System
단백질 태그
GST Tag, His Tag (6x), Untagged
클로닝 방법
Gateway
수량
20 reactions
벡터
pDEST, Gateway Baculovirus Vectors
제품라인
Gateway™
구성 및 보관
The Baculovirus Expression System with Gateway™ Technology includes LR Clonase™ enzyme mix and reaction buffer, destination vectors pDEST™8, pDEST™10, and pDEST™20, proteinase K solution (2 μg/μl), pENTR™-gus positive control, and Library Efficiency™ DH5Competent Cells. The kit does not include MAX Efficiency™ DH10Bac™ Competent Cells. Store LR Clonase™ enzyme mix and Competent Cells at -80°C. Store all other components at -20°C. Components are guaranteed stable for 6 months when properly stored.