Invitrogen™

PureLink™ RNA Mini Kit

카탈로그 번호: 12183020
Invitrogen™

PureLink™ RNA Mini Kit

카탈로그 번호: 12183020
카탈로그 번호
12183020
제품 사이즈
10 preps
제품 정가(KRW)
제품 정가 193,000
공급가
재고 정보
-
수량
카탈로그 번호
제품 사이즈
50 Preps
제품 정가(KRW)
제품 정가 388,000
온라인 행사 350,000
(01-Jul-2024에 종료)
공급가
재고 정보
-
수량
카탈로그 번호
제품 사이즈
250 preps
제품 정가(KRW)
제품 정가 1,949,000
공급가
재고 정보
-
수량
카탈로그 번호제품 사이즈제품 정가(KRW)재고 정보수량
1218302010 preps
제품 정가 193,000
공급가
-
12183018A50 Preps
제품 정가 388,000
온라인 행사 350,000
(01-Jul-2024에 종료)
공급가
-
12183025250 preps
제품 정가 1,949,000
공급가
-
제품 개요
그림
동영상
관련 제품 추천
관련 제품 추천
문서
FAQ
인용 및 참조 문헌
추가 정보
관련 제품 추천
The PureLink RNA Mini Kit is a column-based kit used to isolate high-quality total RNA from a wide variety of sample types in 20 minutes using standard laboratory equipment. The kit includes RNase-free lysis and wash solutions that protect RNA from RNases while liberating the RNA from DNA, proteins, and other cellular debris. The fast spin-column workflow is ideal for processing low to mid-throughput batch sizes. The advanced PureLink RNA Mini spin column design allows for maximum sample input (200 mg of tissue) and RNA recovery (up to 1000 μg). This means that users can process both small and large sample sizes with the same RNA isolation kit.

• Process sample inputs from 5–200 mg of animal/plant tissue or from 0.5 x 106 to 108 tissue culture cells with one spin column in 20 minutes
• Isolate more RNA than competitor mini spin columns: up to 1000 μg of total RNA from one column
• Use isolated RNA in most applications without the need for further DNase treatment (very low minimal residual DNA carry over)
• Flexible–optional on-column or post-isolation DNase treatment is available with PureLink DNase Set for easy removal of residual DNA

Extra-large binding capacity enables rapid RNA purification using standard laboratory equipment
The PureLink RNA Mini Kit provides rapid purification of total RNA from a wide range of cells and tissue types to yield up to 1000 μg of purified RNA from a single extraction (see Figure 1). High-quality total RNA can be obtained from mini- to midi-prep amounts of starting material with just trace amounts of residual genomic DNA contamination. The extra-large binding capacity enables one kit to handle most RNA isolation needs in a quick 20 minute protocol without the need for special sample processing instruments.

Easy, optional, on-column DNase treatment for sensitive applications
The PureLink RNA Mini Kit columns are highly efficient for isolating high quality total RNA while removing the majority of genomic DNA. In general, most applications requiring RNA from animal tissue or mammalian cell lines do not require additional DNase treatment. However, some applications such as gene expression analysis by qRT-PCR without intron-spanning primers or working with samples from organisms with very small or no introns may require more complete removal of residual contaminating DNA. The PureLink DNase Set allows for convenient on-column digestion of DNA during the RNA isolation protocol. Treating with DNase while 'on-column' is easier and allows higher RNA recovery than treating with DNase after the RNA has been isolated. The PureLink DNase Set can also be used to remove residual DNA from RNA that has been previously purified. Both the 'on-column' and post-RNA purification workflows are options available with the PureLink DNase Set.

Simplified, non-toxic RNA purification
The PureLink RNA Mini Kit utilizes non-toxic guanidine-isothiocyanate lysis buffer to protect the RNA during the isolation steps. Special RNase-free reagents combined with certified RNase-free silica membranes in a unique column configuration allow for a safe and easy procedure that can typically be completed in less than 20 minutes without the need of hazardous phenol/chloroform extraction, CsCl centrifugation, or LiCl or alcohol precipitation.

The PureLink RNA Mini Kit is recommended for use with the Homogenizer (Cat. No. 12183026), designed to homogenize cell or tissue lysates via centrifugation, prior to nucleic acid purification. The Homogenizer is especially effective for clarifying particulates from plant tissues.
For Research Use Only. Not for use in diagnostic procedures.

사양

Isolation Technology
Silica Spin Column
정제 시간
20 min. (After sample homogenization)
용리량
30–300 μL
샘플 종류
Bacteria, Blood, Cells, Plant, Tissue, Yeast, Enzymatic Reactions
최종 제품 유형
Total RNA
고처리량 호환성
Not High-throughput Compatible (Manual)
용도(애플리케이션)
RT-PCR, qPCR, cDNA Library Construction, NGS, Microarray Analysis, Blot Hybridization, Northern Blotting, In Vitro Translation, Nuclease Protection Assays, Nucleic Acid Labeling
수량
10 Preps
스케일
Mini
배송 조건
Room Temperature
시작 물질 양
Bacteria: ≤109 cells
Blood: ≤0.2 mL
Cells: ≤5 x 107
Plant: ≤250 mg
Tissue: ≤200 mg
Yeast: ≤5 x 108 cells
Enzymatic reactions: ≤1.2 mL
수율
Up to 350 μg

구성 및 보관

• 20 mL Lysis Buffer; store at room temperature
• 10 mL Wash Buffer I; room temperature
• 4 mL Wash Buffer II; room temperature
• 3 mL RNase-free Water; room temperature
• 10 PureLink Spin Cartridges with Collection Tubes; room temperature
• 10 Collection Tubes; room temperature
• 10 Recovery Tubes; room temperature

그림

문서 및 다운로드

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