The pcDNA™ vectors are designed for high-level, constitutive expression in a variety of mammalian cell lines. The Gateway™ pcDNA™-DEST47 vector offers the following key features:
•C-terminal Cycle 3 GFP tag for rapid detection of recombinant protein •Cytomegalovirus (CMV) promoter for high-level expression •attR sites for Gateway™ cloning, enabling recombination with attL-flanked fragments •Neomycin resistance gene for stable selection •Ampicillin resistance gene and pUC origin for selection and maintenance in E. coli
Gateway™ Cloning To fit all of your expression needs, Invitrogen offers state-of-the-art Gateway™ destination vectors for expression in E. coli, insect, yeast, or mammalian cells, as well as for production of native protein or N- or C-terminal fusion proteins. All Gateway™ destination vectors have attR sites for recombination with any attL-flanked fragment, regardless of whether it is an entry clone or an Ultimate™ ORF Clone. The following table lists a variety of available destination vectors.
Additional materials required, available separately: Gateway™ entry clone, appropriate Gateway™ LR Clonase™ enzyme mix, and reaction buffer.
For Research Use Only. Not for use in diagnostic procedures.
사양
구성 또는 유도성 시스템
Constitutive
배달 유형
Transfection
프로모터
CMV
제품 유형
Gateway System Destination Expression Vector
리포터 유전자
GFP (Cycle 3)
선택 제제(진핵)
Geneticin™ (G-418)
단백질 태그
GFP (Cycle 3)
클로닝 방법
Gateway
벡터
pDEST, pcDNA
제품라인
Gateway™, pDEST™
용도(애플리케이션)
Constitutive Expression
구성 및 보관
All destination vectors are provided lyophilized and supercoiled.