Thermo Scientific™ Pierce Nickel Coated Plates provide a simple format to bind His-tagged fusion proteins for ELISA and other plate-based assays involving protein interactions of expressed recombinant proteins.
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설명
검출 방법
15242
White, 96-well
Chemiluminescence
15342
Black, 96-well
Fluorescence
15142
Clear, 8-well Strip
Colorimetric
15442
Clear, 96-well
Colorimetric
카탈로그 번호 15242
제품 정가(KRW)/ 5 plates
제품 정가414,000
온라인 행사332,000온라인 주문시에만 적용 (Supply Center 제외) .
(ends 01-Oct-2024)
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설명:
White, 96-well
검출 방법:
Chemiluminescence
대량 주문 또는 맞춤형 요청
제품 정가(KRW)/ 5 plates
온라인 행사332,000온라인 주문시에만 적용 (Supply Center 제외) .
(ends 01-Oct-2024)
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Pierce™ Nickel Coated Plates
카탈로그 번호15242
제품 정가(KRW)/ 5 plates
온라인 행사332,000온라인 주문시에만 적용 (Supply Center 제외) .
(ends 01-Oct-2024)
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These Ni(2+) chelate-coated plates are ideal for analyzing polyhistidine-tagged fusion proteins by ELISA-based methods. Proteins that contain a succession of several histidine residues at the amino or carboxyl terminus have a strong binding affinity for metal. Bacterial lysates containing polyhistidine-tagged fusion proteins can be added directly to the plates without the need for blocking. The clear, white or black plates can be used with colorimetric, chemiluminescent or fluorescent detection methods, respectively.
Thermo Scientific Pierce Nickel Coated White 96-Well Plates provide a simple format to bind His-tagged fusion proteins for ELISA and other plate-based assays involving protein interactions of expressed recombinant proteins. These white plates can be used with chemiluminescent detection methods.
Features of Pierce Nickel Coated Plates include: • Ni2+ activated surface enables metal-chelate binding of His-tagged proteins • Detergents used to lyse cells don't inhibit binding to activated plates as they do with plain polystyrene • Better binding for sensitive assays compared to other commercially available nickel-activated plates • Detection limit: 1 ng of polyhistidine fusion protein • Binding capacity: approx. 9 pmol His-tagged protein (27 kDa) per well • Activation level: 200 μL
These Ni(2+) chelate-coated plates are ideal for analyzing polyhistidine-tagged fusion proteins by ELISA-based methods. Proteins that contain a succession of several histidine residues at the amino or carboxyl terminus have a strong binding affinity for metal. Bacterial lysates containing polyhistidine-tagged fusion proteins can be added directly to the plates without the need for blocking.
For Research Use Only. Not for use in diagnostic procedures.
사양
결합 특성Standard Capacity, Affinity
설명White, 96-well
검출 방법Chemiluminescence
포장5 Plates
제품라인Pierce
색상White
제품 유형Microplate
물질Polystyrene
부피(미터법) 웰200 μL
웰 수96
표면 처리Divalent Nickel, Metal Chelate
플레이트 블로킹Blocker™ BSA Buffer
Unit Size5 plates
구성 및 보관
Upon receipt store plates at 4°C in unopened pouches. Once opened, place unused plates in a resealable bag with desiccant and store at 4°C.