Thermo Scientific Pierce SIA is a very short (1.5 angstrom) crosslinker for amine-to-sulfhydryl conjugation via N-hydroxysuccinimide (NHS) ester and iodoacetyl reactive groups.
Features of SIA:
• Reactive groups:NHS ester and iodoacetyl • Reactive toward: amino and sulfhydryl groups • Shortest sulfhydryl-reactive and amine-reactive heterobifunctional crosslinker available; compare to SIAB • NHS ester reacts with primary amines at pH 7-9 to form a stable amide bond • Iodoacetyl group reacts with sulfhydryl groups at pH > 7.5 to form stable thioether bond • Non-cleavable • Water-insoluble (dissolve first in DMF or DMSO)
Product References:
Crosslinker Application Guide -- search for recent literature references for this product
For Research Use Only. Not for use in diagnostic procedures.
사양
화학물질 반응성
Amine-Sulfhydryl
Cleavable
No
설명
SIA
분자량
283.02
페길레이티드
No
스페이서 암 길이
1.5 Å
세포 투과성
Yes
배송 조건
Ambient
제품라인
Pierce™
라벨링 방법
Chemical Labeling
교차결합제 유형
Heterobifunctional
반응성 부분
Haloacetyl, NHS Ester
스페이서
Short (<10 Å)
형태
Solid
수량
50 mg
용해도
DMF, DMSO
형식
Standard
수용성
No
제품 유형
Crosslinker
구성 및 보관
Upon receipt store at 4°C protected from moisture.