Easy-Titer™ Human IgM Assay Kit
Product Image
Thermo Scientific™

Easy-Titer™ Human IgM Assay Kit

The Thermo Scientific Easy-Titer Human IgM Assay Kit includes antibody-sensitized microspheres to measure the specific concentration of antibodies by an자세히 알아보기
Have Questions?
카탈로그 번호수량
2331596 Tests
카탈로그 번호 23315
제품 가격(KRW)
627,000
Online offer
Ends: 30-Sep-2025
737,000
할인액 110,000 (15%)
Each
카트에 추가하기
수량:
96 Tests
제품 가격(KRW)
627,000
Online offer
Ends: 30-Sep-2025
737,000
할인액 110,000 (15%)
Each
카트에 추가하기
Ask our AI about this Product
The Thermo Scientific Easy-Titer Human IgM Assay Kit includes antibody-sensitized microspheres to measure the specific concentration of antibodies by an easy and rapid microagglutination technique using standard microplates and UV-Vis plate reader (spectrophotometer). This kit is specific for human IgM and, unlike total protein assays, can specifically measure the concentration of target antibody in samples (e.g., serum, plasma, culture supernatant) that contain other proteins. It is sensitive, requiring very small sample volumes. Antibody concentration is determined from the assay response (absorbance) by comparison to a standard curve prepared using dilutions of a known antibody sample (sold separately).

General features of Easy-Titer Antibody Assay Kits:

Antibody-based specificity—measure concentration of target antibody in a sample, not just total protein; no need to purify antibody to assess its concentration
Faster and easier than ELISA—three-component, homogenous assay; 10 minutes total incubation time
No special equipment needed—uses standard vortex mixer, pipetter, 96-well microplate, plate shaker and reader (measure absorbance at 340nm or 400nm)
Sensitive—assay range (standard curve) 8 to 500 ng/mL; use sample at 15 to 300 ng/mL for optimal results
Reproducible—coefficient of variation less than 5%; error depends on dilution and pipetting technique
Antibody standards sold separately—see product list for suggested products; use any antibody standard with proper target identity and known concentration (greater than 10 μg/mL)
Kits for five popular targets—choose a kit specific for a particular species and class of immunoglobulin; no cross-reaction with other species and classes of the target antibody

Easy-Titer Assay Kits detect and measure specific target antibodies using agglutination of microspheres that are coated ('sensitized') with the specific anti-IgG or IgM polyclonal antibodies. In the appropriate aqueous buffer (supplied in kit), the monodispersed antibody-coated microspheres (> 1 μM diameter) have highest absorptivity (λ-max) to incident light having a wavelength (340nm) that is equal to approximately half their diameter. When sample is added, two or more microspheres bind to each antibody target via their coated specific polyclonal antibodies, and this agglutination into effectively larger apparent spheres results in proportional decrease in absorptivity (lower absorbance).

Typical microagglutination assays depend on a change in light-scattering and corresponding change in transmittance, to which absorbance is inversely related. Easy-Titer Assay Kits use a special dilution buffer whose refractive index eliminates the effect of light-scattering on the monodispersed microspheres for the measurement wavelength used. Because of this, the final 10- to 20-fold dilution of the sample for use in the assay must be done using the Dilution Buffer supplied in the kit.

Easy-Titer Antibody Assays are faster and easier than ELISA:
• Prepare standards (5 to 500 ng/mL) by diluting purified antibody in Kit Dilution Buffer.
• Prepare samples by diluting in Dilution Buffer to within assay range (8 to 500 ng/mL).
• Vortex vial of microsphere beads to create homogeneous suspension.
• Pipette 20 μL of bead suspension and 20 μL of each sample and standard into 96-well microplate wells.
• Incubate microplate for 5 minutes with vigorous mixing.
• Add 100 μL of Kit Blocking Reagent.
• Incubate microplate for 5 minutes with vigorous mixing.
• Measure absorbance on standard plate reader (340nm or 405nm).
• Plot standard curve and interpolate samples to determine concentration.

Related Products
Easy-Titer™ Mouse IgG Assay Kit
Easy-Titer™ Rabbit IgG Assay Kit
Easy-Titer™ Human IgG (H+L) Assay Kit
Easy-Titer™ Human IgG (gamma chain) Assay Kit
For Research Use Only. Not for use in diagnostic procedures.
사양
설명Easy-Titer Human IgM Assay Kit
용도(애플리케이션)Solution-based Detection, Absorbance
용도(장비)Spectrophotometer, Microplate Reader
수량96 Tests
어세이Protein Quantitation Assay, Isotyping Assay, Immunoassay
검출 방법Colorimetric
제품라인Easy-Titer™
타겟 특이도Target-specific
Unit SizeEach
구성 및 보관
Sufficient For: 96 total tests (including 9 standards per use)
• Goat Anti-Human IgM Sensitized Polystyrene Beads, 2mL
• Easy-Titer Dilution Buffer, 30 mL
• Easy-Titer Blocking Buffer, 15 mL

Store at 4°C (do not freeze).
Have questions about this product? Ask our AI assisted search.
This is an AI-powered search and may not always get things right. You can help us make it better with a thumbs up or down on individual answers or by selecting the “Give feedback" button. Your search history and customer login information may be retained by Thermo Fisher and processed in accordance with our Privacy Notice.

문서 및 다운로드

Certificates | 증명서

Safety Data Sheets | 물질 안전 보건 자료

카탈로그 번호, 제품명 또는 링크 공유