Sufficient For: 30 chromatin preparations for ChIP assays
• Membrane Extraction Buffer, 10 mL
• Nuclear Extraction Buffer, 10 mL
• MNase Digestion Buffer, 5 mL
• MNase Stop Solution, 0.5 mL
• Halt Protease and Phosphatase Inhibitor Cocktail EDTA-free (100X), 4 x 90 μL
• Sodium chloride (5M), 3 mL
• Glycine solution (10X), 15 mL
• PBS (20X), 15 mL
• Micrococcal Nuclease (ChIP Grade) (10 U/μL) (store at -20°C)
• Proteinase K (20 mg/mL), 0.25 mL (store at -20°C)
• DTT, Lyophilized, 1 vial

Unless otherwise indicated, store all components at 4°C.