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카탈로그 번호 | 제품 사이즈 | 제품 정가(KRW) | 재고 정보 | 수량 | |
---|---|---|---|---|---|
33033 | 10 mg | 제품 정가 556,000 온라인 행사 444,000 (01-Oct-2024에 종료) 공급가 | - | ||
A39260 | 제품 정가 671,000 온라인 행사 571,000 (01-Oct-2024에 종료) 공급가 | - |
Typical protocol for label transfer experiment:
• Add a few microliters of dissolved Sulfo-SBED Reagent to 0.5-1 mL of purified bait protein in PBS.
• Incubate mixture for 30-120 minutes on ice or at room temperature in the dark.
• Desalt or dialyze (in subdued light) to remove excess non-reacted Sulfo-SBED from the labeled bait protein.
• Add labeled bait protein to cell lysate or other solution containing putative target protein interactors ('prey').
• When interaction complexes have formed, expose the solution to ultraviolet light (365 nm) for several minutes.
• Analyze products by one of several methods:
• Western Blotting: Cleave crosslinks in DTT, separate proteins by SDS-PAGE, and detect biotinylated bands by Western blotting with streptavidin-HRP.
• Purification and Mass Spec or Sequencing: Affinity-purify biotinylated proteins or peptide fragments following trypsin digestion and perform MS or sequencing to characterize the proteins involved.
Related Products
Sulfo-SBED Biotin Label Transfer Kit - Western Blot Application