TaqMan™ QSY and QSY2 Probes

Applied Biosystems TaqMan QSY and QSY2 probes are dual-labeled probes used for real-time PCR with Cyanine 5 (Cy5) and Cyanine 5.5 (Cy5.5) reporter dyes. TaqMan QSY and QSY2 Probes are designed for optimal sensitivity and performance in multiplexed qPCR experiments for detection of up to 6 targets. Current users of dark quencher probes, such as Black Hole Quencher (BHQ™) probes, can design custom QSY and QSY2 probes without changing probe sequences.

All TaqMan QSY and QSY2 probes are HPLC-purified and verified by mass spectrometry to help ensure lot-to-lot reproducibility between batches. Thermo Fisher Scientific has been a leader in qPCR probe manufacturing for over 20 years, with expertise in probe synthesis in a facility that is certified to ISO 13485. TaqMan QSY and QSY2 probes can be used for viral pathogen and microbial detection in research samples for healthcare and biopharmaceutical development needs.

Features of TaqMan QSY and QSY2 probes include:

• Cyanine 5 (Cy5) and Cyanine 5.5 (Cy5.5) reporter dyes—new TaqMan QSY2 probes include Cy5 and Cy5.5 reporter dyes, now enabling you to multiplex up to six targets
• Increased sensitivity compared to probes from another leading supplier—detect low-abundance targets, impurities, and fluctuations in bioproduction more accurately
• Multiplexing performance without compromise—use QSY for multiplexing up to four targets with ABY, JUN, FAM, and VIC and use QSY2 for 5th and 6th target multiplexing with Cy5 and Cy5.5
• Better performance retention when scaling up—consistent performance from singleplex to 6-plex creates a smoother assay development experience
• TaqMan QSY and QSY2 probes have greater dynamic range compared to probes from another leading supplier—enables more accurate detection of a greater range of analyte concentrations
• Get started quickly—BHQ probe sequences can be converted to QSY and QSY2 versions with no redesign needed
• Compatible with our master mixes and validated on our instruments—get increased confidence in the performance of the probes in your lab to help get your products to market faster
• Part of the Thermo Fisher Scientific qPCR ecosystem—our teams can support you in all components of the real‐time PCR (qPCR) workflow

Guaranteed yield

The unit size you select is based on the minimum amount we will deliver, not on synthesis starting amount. Ensure that you are considering the guaranteed yield (amount of probe you are actually receiving) when calculating price per reaction.

Approximate ship times

• 4 to 10 days in North America
• 5 to 10 days in Europe

Calibration

For calibration of ABY, JUN, and Cy5, the complete list of calibration plates for 96-well, 0.1 mL; 96-well, 0.2 mL; and 384-well plate formats are in the Multiplexing Optimization Guide in the document section. For Calibration of Cy5.5, please email your request to specialty_oligos@thermofisher.com.

We also offer Applied Biosystems custom primers and other types of TaqMan probes.

Need a different dye or concentration or a larger lot size? The TaqMan Custom Assay and Oligo Service offers custom products that you can tailor to meet your experimental needs.