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SMART Digest™ ImmunoAffinity (IA) Streptavidin Kits
Obtain fast, simple, highly reproducible, and sensitive streptavidin immunocapture and trypsin protein digestion in a single well for your biomarker studies.
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| 카탈로그 번호 | 포함 |
|---|---|
| 60110-101 | SMART Digest IA Kit, Streptavidin (Av) non-magnetic |
| 60110-102 | SMART Digest IA Kit, Av non-magnetic with SOLAμ SPE and collection plate |
| 60110-103 | SMART Digest IA Kit, Av magnetic with SOLAμ SPE and collection plate |
| 60110-104 | SMART Digest IA Kit, Av magnetic |
| 60110-104-NW-10PK | Bulk Magnetic beads with immobilized streptavidin and trypsin for capture and digestion and SMART Digest buffer |
카탈로그 번호 60110-101
제품 가격(KRW)Contact Us
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포함:
SMART Digest IA Kit, Streptavidin (Av) non-magnetic
Obtain fast, simple, highly reproducible, and sensitive immunocapture and protein digestion in a single well using Thermo Scientific™ SMART Digest™ ImmunoAffinity (IA) Streptavidin Kits. These kits were designed to remove the challenges often associated with the process of biomarker quantitation by providing a simple and easy capture-digestion workflow that takes only hours instead of overnight. The kit purifies various target biomolecules via the formation of strong streptavidin-biotin bond.
Solving the Challenges of Low-level Quantitation in Complex Matrices
The need to improve the ability to identify low-level biomarkers is forcing the analytical community to deal with extremely high levels of analyte and sample complexity, which makes quantitation challenging.
As these proteins are often present at low levels in complex biological matrices, an immunoaffinity capture step is typically employed to purify the sample and increase sensitivity. This step is often followed by protein digestion. Current processes are often time consuming, multistep, and prone to irreproducibility.
Immunocapture and Protein Digestion in a Single Well
These IA kits deliver a process that is:
- Fast
- Simple
- Highly reproducible
- Sensitive
Unique Design Reduces Workflow to Hours
In SMART Digest IA Kits, the immunocapture reagents and the temperature-activated, thermally stable trypsin are co-immobilized onto a resin. Following enrichment, the enzyme is activated at elevated temperatures for accelerated digestion under protein denaturing conditions, resulting in a workflow which takes hours rather than overnight.
사양
용도(애플리케이션)Peptide Quantitation
포함SMART Digest IA Kit, Streptavidin (Av) non-magnetic
CaptureStreptavidin
CleavageC-terminal side of lysine and arginine amino acids
효소Trypsin
형식SMART Digest Resin, Non-magnetic
제품라인SMART Digest
유형Immunocapture + Protein Digestion combined on a single bead
Unit SizeEach
Putting Peptides on the Map
How do advances in digestion, separation, mass detection & data interpretation remove roadblocks in protein characterization?
Faster and More Sensitive Protein Characterization and Quantitation
How can characterization and quantitation of bio-therapeutics can be made easier, faster and more reproducible?
Have questions about this product? Ask our AI assisted search.
Why do I need to perform reduction and alkylation post digestion when using SMART Digest ImmunoAffinity (IA) Kits?
Why don't I need to quantitate the total amount of protein and titer the enzyme when using the SMART Digest ImmunoAffinity (IA) Kits?
Can I use surfactants with SMART Digest ImmunoAffinity (IA) Kits?
Is the trypsin mutated to be heat stable in SMART Digest ImmunoAffinity (IA) Kits? Would this affect my sample?
What amount of materials can I digest with the SMART Digest ImmunoAffinity (IA) Kits?
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Product Information
자주 묻는 질문(FAQ)
We have observed the following effects with detergents:
• CHAPS – ~ 30% reduction in digest efficiency
• OGS – No reduction in digestion efficiency
• TWEEN – No reduction in digestion efficiency
• RIPA – The addition of RIPA, for ribonuclease A digestion, results in a concentration-dependent effect, where initial enzyme inhibition is overcome by improved substrate solubilization at higher concentrations only. 20% reduction in digestion efficiency.
We recommend compensating for reduction in digestion efficiency by extending the digestion time accordingly.
The SMART Digest kits are not affected by concentrations of up to 0.5M of urea. If the concentration is higher than this, we recommend that the sample is diluted to <0.5 M of urea, prior to beginning digestion using the SMART Digest kit.
The composition of the SMART Digest IA buffer is as follows:
Chemical Name (CAS No., EINECS No.): Kit Component Qty, Weight %
• Water (7732-18-5, 231-791-2): 2, 50-95%
• Glycerol (56-81-5, 200-289-5): 2, < 20%
• Tris Base (77-86-1, 201-064-4): 2, < 10%
• Tris-HCI (1185-53-1, 214-684-5): 2, < 10%
• Calcium Chloride (10043-52-4, 233-140-8): 2, < 10%
• Sodium Azide (26628-22-8, 247-852-1): 2, < 0.1%
The SMART Digest IA Kits contain an excess of enzyme capable of digesting between 200 pg and 3.5 mg of protein. As most samples will fall within this range, it is not necessary to routinely quantitate protein concentration prior to digestion.
The reason urea is needed as a first step in an in-solution protocol is to disrupt the sample and partially unfold the proteins. The proteins need to be partially unfolded so that the trypsin enzyme can have better access to the internal amino acid chain, not just the surface of the protein of interest. The reason the SMART Digest IA kits do not need urea is that it uses heat to unfold the protein.