The PrioCHECK® CSFV Antibody ELISA is a blocking ELISA for the detection of antibodies directed against CSFV in pigs. The test detects antibodies against high-, moderate-, and low-virulent CSFV strains early after infection. The PrioCHECK® CSFV Antibody ELISA uses two monoclonal antibodies (mAbs) to measure the binding of CSFV antibodies in the test sample directed against the E2 protein of CSFV. One mAb is bound to the test plate; the other, the detection antibody, is conjugated to an enzyme that generates a color signal. The signal is then measured and when no color is formed, the sample containing anti-CSFV antibodies that have competed for the available antigen is positive for CSFV.