Thermo Scientific SuperSignal Enhanced Molecular Weight Protein Ladder (20 to 150K) is a mix of eight unstained recombinant proteins with IgG-binding sites for chemiluminescent, fluorescent, chromogenic or other detection systems. The protein ladder is supplied in a ready-to-use format for direct loading onto gels; no need to heat, reduce, or add sample buffer prior to use.
The ladder’s recombinant proteins bind to the antibodies used in the western blot through the IgG binding site. The protein markers can then be visualized using appropriate substrates for enzyme-labeled antibodies or via fluorescent dye-labeled antibodies (not recommended for antibodies labeled with fluors in the 500–550 nm channel).
Two versions of the MW marker are offered. This formulation should be used in applications requiring mouse monoclonal antibodies and experiments where very dilute antibody concentrations are used. For example, the enhanced standard may be necessary when using a 1:50,000 (25 ng/mL) to 1:100,000 (10 ng/mL) dilution of secondary antibody. The SuperSignal Molecular Weight Protein ladder formulation is appropriate for use with most rabbit and other non-mouse polyclonal antibodies.
사양
설명
SuperSignal Enhanced Molecular Weight Protein Ladder
검출 방법
User defined detection system
Number of Markers
8
제품 유형
Protein Ladder
로드 준비
Yes
크기 범위
20 to 150 kDa
염색 유형
Unstained
분자량
150, 100, 80, 60, 50, 40, 30, 20 kDa
제품라인
SuperSignal™
수량
4 x 250 μL
시스템 유형
SDS-PAGE
구성 및 보관
Contents: four vials of 250 μL
Storage buffer: 62.5 mM Tris-H3PO4 (pH 7.5 at 25°C), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN3 and 33% glycerol
Storage: Upon receipt store at -20°C for long term storage, or three months at 4°C or one month at ambient