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Pierce™ High pH Reversed-Phase Peptide Fractionation Kit
Pierce™ High pH Reversed-Phase Peptide Fractionation Kit
Thermo Scientific™

Pierce™ High pH Reversed-Phase Peptide Fractionation Kit

Increase protein identification from LC/MS analysis through orthogonal peptide fractionation of complex peptide samples using Thermo Scientific™ Pierce™ High pH Reversed-Phase Peptide Fractionation Kit.
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카탈로그 번호수량
8486812 Reactions
카탈로그 번호 84868
제품 가격(KRW)
486,000
Each
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수량:
12 Reactions
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제품 가격(KRW)
486,000
Each
카트에 추가하기
In order to enable deep proteome sequencing, it is often necessary to reduce the sample complexity by fractionation in an orthogonal dimension prior to LC/MS analysis. The Pierce High pH Reversed-Phase Peptide Fractionation Kit utilizes high pH reversed-phase chromatography to separate peptides by hydrophobicity, and provides excellent orthogonality to low pH reversed-phase LC-MS gradients. The kit has been designed to improve protein identification through the use of a proprietary reversed-phase resin in an easy-to-use spin column format with a high-pH fractionation protocol.

Thermo Scientific Pierce High pH Reversed-Phase Peptide Fractionation Kit increases protein identification from LC/MS analysis through orthogonal peptide fractionation of complex peptide samples.

  • Easy-to-use - resin provided in single-use spin column format
  • Improved protein identifications - protein identifications increased by ≥50% when compared to unfractionated samples
  • Reproducible - elution profiles and fractional resolution vary by less than 20%
  • Optimized - robust procedure for maximal protein identification and peptide recovery while minimizing fractional overlap
  • Compatible - reagents validated with a variety of complex samples, including TMT™-labeled peptides
In order to enable deep proteome sequencing, it is often necessary to reduce the sample complexity by fractionation in an orthogonal dimension prior to LC/MS analysis. The Pierce High pH Reversed-Phase Peptide Fractionation Kit utilizes high pH reversed-phase chromatography to separate peptides by hydrophobicity and provides excellent orthogonality to low pH reversed-phase LC-MS gradients.
The kit has been designed to improve protein identification through the use of a proprietary reversed-phase resin in an easy-to-use spin column format with a high-pH fractionation protocol. In contrast to strong cation exchange (SCX) fractionation, the high-pH reversed-phase fractions do not require an additional desalting step before LC/MS analysis.
The High pH Reversed-phase Peptide Fractionation Kit includes a high pH buffer (0.1% triethylamine) and twelve spin columns containing pH-resistant reversed-phase resin. Each reversed-phase fractionation spin column enables fractionation of 10 to 100μg of peptide sample using a microcentrifuge.
Native, phosphorylated, Tandem Mass Tag™ (TMT)-labeled, and other complex peptide mixture samples can be fractionated using this kit. Combining the search results generated by the individual fractions helps improve protein sequence coverage and increases the number of identified proteins relative to unfractionated samples.
Applications:
  • Reducing sample complexity to identify targets of interest
  • Conducting systems biology studies
  • Reducing sample complexity to improve quantitation studies

For Research Use Only. Not for use in diagnostic procedures.
사양
최종 제품 유형Peptides
용도(장비)Microcentrifuge
수량12 Reactions
워크플로우 단계Fractionation
검출 방법Mass Spectrometry
형식Kit
제품라인Pierce™
제품 유형Peptide Fractionation Kit
시작 물질Peptides, Protease-digested Protein
Unit SizeEach
구성 및 보관
Reversed-phase fractionation spin columns, 12 columns
Triethylamine (0.1%), 100 mL

Store in refrigerator (2–8°C).

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