Pierce™ Biotin 3' End DNA Labeling Kit
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Pierce™ Biotin 3' End DNA Labeling Kit
Thermo Scientific™

Pierce™ Biotin 3' End DNA Labeling Kit

The Thermo Scientific Pierce Biotin 3' End DNA Labeling Kit is for tagging single-stranded DNA primers with biotin for use자세히 알아보기
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8981820 Reactions
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The Thermo Scientific Pierce Biotin 3' End DNA Labeling Kit is for tagging single-stranded DNA primers with biotin for use in non-radioactive electrophoretic mobility shift assays (EMSA) and other nucleic acid detection methods.

The DNA biotinylation procedure uses terminal deoxynucleotidyl transferase (TdT) to catalyze nontemplate-directed nucleotide incorporation onto the 3'-OH end of single-stranded DNA. TdT exhibits a substrate preference of single-stranded DNA, but it will label duplex DNA with 3' overhangs and blunt duplexes, albeit with a lower efficiency.

Features of the Biotin 3' End DNA Labeling Kit:

• Non-isotopic labeling eliminates the hassle of hazardous radioactive materials or difficult-to-dispose-of waste
• 1-3 biotinylated ribonucleotides onto the 3' end of DNA strands for less interference with hybridization or sequence-specific binding of proteins
• Biotin-labeled probes are stable for more than one year
• 30-minute labeling procedure is fast and efficient

The Biotin 3' End DNA Labeling Kit has been optimized to incorporate 1-3 biotinylated ribonucleotides (Biotin-11-UTP) onto the 3' end of DNA strands. This labeling strategy has the advantage of localizing the biotin to the 3' end of the probe where it will be less likely to interfere with hybridization or sequence-specific binding of proteins. Biotin-labeled DNA probes can be used to facilitate non-isotopic detection in a variety of applications including electrophoretic mobility shift assays (EMSA), Northern or Southern blots, colony hybridizations or in situ hybridizations.
For Research Use Only. Not for use in diagnostic procedures.
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반응 수20
제품라인Pierce™
제품 유형DNA Labeling Kit
수량20 Reactions
형식Kit
Labeling TargetDNA
Unit SizeEach
구성 및 보관
Sufficient For: 20 labeling reactions (each with 5 pmol DNA)
• 1 mL 5X TdT Reaction Buffer (-20°C)
• 5 μL Terminal Deoxynucleotidyl Tranferase (-20°C)
• 1 μL Biotin-11-UTP (≤4°C)
• 14 μL Unlabeled Control Oligo (-20°C)
• 4 μL Biotin-Control Oligo (-20°C)
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Certificates | 증명서

Lot 번호Certificate TypeDateCatalog Number(s)
AC402602Certificate of Analysis2025년 3월 27일89818
ZF391991Certificate of Analysis2024년 7월 10일89818
ZC375713Certificate of Analysis2024년 3월 09일89818
YJ381940Certificate of Analysis2023년 10월 18일89818
YE372382Certificate of Analysis2023년 5월 24일89818
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