Gateway™ entry vectors are designed to clone DNA sequences using restriction endonucleases and ligase to create a Gateway™ entry clone. The resulting entry clone is ready for recombination with a destination vector to create an expression clone. New: pENTR™ Dual Selection vectors!
The Gateway™ entry vectors (Table 1) offer the following: • attL1 and attL2 sites for site-specific recombination of the entry clone with a Gateway™ destination vector to ensure cloning of the gene of interest in the correct orientation for expression • Kozak consensus sequence for efficient translation initiation in eukaryotic systems • Ribosome binding site for efficient translation initiation in prokaryotic systems (pENTR™ 1A Dual Selection, pENTR™3C Dual Selection, and pENTR™11 Dual Selection vectors only) • rrnB transcription termination sequences to prevent basal expression of the PCR product of interest in E. coli • pUC origin for high-copy replication and maintenance of the plasmid in E. coli • Kanamycin resistance gene for selection in E. coli • The ccdB⁄chloramphenicol fusion gene located between the two attL sites for o negative selection and o Chloramphenicol selection in E. coli • Kanamycin resistance gene for selection in E. coli
For Research Use Only. Not for use in diagnostic procedures.
사양
제품 유형
Dual SelectionExpression Vector
항생제 내성 박테리아
Chloramphenicol (CmR), Kanamycin (KanR)
분열
No Cleavage Site
단백질 태그
Untagged
클로닝 방법
Gateway
벡터
pENTR
제품라인
pENTR™
구성 및 보관
10 μg pENTR™ Dual Selection vector, in 20 ul in TE buffer, pH 8.0. Store at -20°C