The PolarScreen™ ER (Estrogen Receptor) Alpha Competitor Assay Kit, Green, provides a sensitive and efficient method for high-throughput, fluorescence polarization-based자세히 알아보기
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수량
A15882
2500 Assays
A15883
800 Assays
카탈로그 번호 A15882
제품 정가(KRW)/ 2,500 assays
제품 정가3,543,000
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수량:
2500 Assays
제품 정가(KRW)/ 2,500 assays
제품 정가3,543,000
카트에 추가하기
PolarScreen™ ER Alpha Competitor Assay, Green
카탈로그 번호A15882
제품 정가(KRW)/ 2,500 assays
제품 정가3,543,000
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카트에 추가하기
The PolarScreen™ ER (Estrogen Receptor) Alpha Competitor Assay Kit, Green, provides a sensitive and efficient method for high-throughput, fluorescence polarization-based screening of potential ER alpha ligands. The kit uses insect cell-expressed, full-length, native (untagged) human estrogen receptor alpha (available separately, Cat. No. A15674) and a novel, high-affinity, fluorescent estrogen ligand (Fluormone ES2 Green) in a homogenous, mix-and-read assay format.
This kit produces a green color read-out; a 'red' kit is also available. Both kits suitably determine the IC50s of competitive compounds. Color choice depends on the spectral properties of the compounds to be screened and the available instrumentation. For additional information or assistance contact drugdiscoverytech@lifetech.com.
PolarScreen™ ER Alpha Competitor Assay, Green, is:
• Complete & ready-to-use out of the box—all reagents included, just add your test compounds • High throughput compatible—800 x 20 μL fluorescence polarization assays in a 384-well plate • Convenient—available in bulk sizes for larger screening needs
How the Assay Works Full length ER alpha is added to a fluorescent estrogen ligand to form an ER-Fluormone ES2 complex. This complex is added to individual test compounds in multiwell plates. To determine relative affinity, the assay measures fluorescence polarization. Competing test compounds will displace the Fluormone ES2 ligand from ER alpha, permitting it to tumble rapidly and resulting in low polarization values. A shift in the fluorescence polarization value in the presence of test compound is used to determine the relative affinity of test compounds for ER alpha.
All components are manufactured under the strictest quality parameters. This product's performance, per the Certificate of Analysis, is guaranteed for 6 months after purchase. Contact us for bulk quantities or for assistance with other nuclear receptor assay needs, from assay development to library screening and beyond.
For Research Use Only. Not for use in diagnostic procedures.
사양
검출 방법Fluorescence
용도(장비)Fluorescent Imager
형식384-well Plate
수량2500 Assays
유형Competitor Assay Kit
Unit Size2,500 assays
구성 및 보관
Contains:
• 4 tubes full-length ER Alpha Recombinant Protein (store at -68 to -85°C) • 1 tube of Fluormone ES2 Green Tracer (store at -5 to -30°C) • 2 bottles of ES2 Screening Buffer (store at -5 to -30°C)