The Tag Sequencing Barcode Set 25-48 provides a set of 24 unique barcode adapters specifically designed for optimal performance in Oncomine™ cfDNA assays. The barcode adapters are compatible with the Ion S5™, Ion PGM™, and Ion Proton™ sequencing systems. When used in combination with kits utilizing tag sequencing technology, this barcode set enables users to pool up to 24 amplicon libraries and to conduct multiplexed next-generation sequencing (NGS) analysis, reducing the sequencing cost per sample.

Key product features:
• Enables multiplexing of numerous amplicon library samples on a single sequencing chip with the use of robust molecular barcodes
• Both sequence-optimized and flow-optimized for equal representation of all barcodes in a pool and more economical multiplexed sequencing runs
• Compatible with library construction kits utilizing tag sequencing technology

Multiplexing with barcode adapters enables higher throughput
Multiplexing with barcoded libraries leads to more cost-effective runs by permitting up to 24 samples per run (or more if combined with Tag Sequencing BC Set 1-24, Cat. No. A31830), significantly decreasing the cost and handling requirements of sequencing. Each barcode was individually tested to minimize representation bias.

Sequence-optimized and flow-optimized for increased performance and efficiency
Barcode adapters contain sequences that are optimized to provide equal representation of all barcodes in a pool; they require a minimal number of flows to interrogate resulting in more economical multiplexed sequencing runs.