The Thermo Scientific SILAC Protein Quantitation Kit (Trypsin), DMEM, is used for the specific analysis of protein expression by mass자세히 알아보기
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카탈로그 번호 A33972
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제품 정가(KRW)/ 1 kit
제품 정가1,413,000
카트에 추가하기
SILAC Protein Quantitation Kit (Trypsin), DMEM
카탈로그 번호A33972
제품 정가(KRW)/ 1 kit
제품 정가1,413,000
카트에 추가하기
The Thermo Scientific SILAC Protein Quantitation Kit (Trypsin), DMEM, is used for the specific analysis of protein expression by mass spectrometry using stable isotope labeling with amino acids in cell culture (SILAC).
Features of the SILAC Protein Quantitation Kit, DMEM, include: • Efficient—up to 100% label incorporation into proteins of living cells • Reproducible—minimizes intra-experimental variability caused by differential sample preparation • Flexible—media deficient in both L-lysine and L-arginine, allowing for more complete proteome coverage through dual amino acid isotope labeling • Compatible—label proteins expressed in a wide variety of mammalian cell lines adapted to grow in DMEM medium, including HeLa, 293T, COS7, U2OS, A549, A431, HepG2, NIH 3T3, Jurkat, and others • High-quality supplements—heavy amino acids with >99% isotope purity; dialyzed FBS tested to help ensure that it is sterile, endotoxin-free, and cell culture compatible
Applications of SILAC Protein Quantitation Kit: • Quantitative analysis of relative changes in protein abundance from different cell treatments • Quantitative analysis of proteins for which there are no antibodies available • Protein expression profiling to study normal vs disease cells • Identification and quantification of hundreds to thousands of proteins in a single experiment
SILAC is a powerful method to identify and quantify relative differential changes in complex protein samples. This approach entails the in vivo metabolic incorporation of 'heavy' 13C- or 15N-labeled amino acids into proteins followed by mass spectrometry (MS) for the accelerated and comprehensive identification, characterization, and quantitation of proteins.
Because peptides labeled with heavy and light amino acids are chemically identical, they co-elute during reverse-phase column pre-fractionation and therefore are detected simultaneously during MS analysis. The relative peak intensities of multiple isotopically distinct peptides from each protein are then used to determine the average change in protein abundance in the treated sample.
Trypsin SILAC kits are supplied with 13C615N2 L-lysine-2HCl and 13C615N4 L-arginine-HCl to label and quantify lysine-containing and arginine-containing peptides from trypsin protein digests. Each kit also includes 2 x 500 mL of medium and dialyzed serum. When combined with Thermo Scientific protein/peptide sample enrichment products, SILAC Protein Quantitation kits also enable MS analysis of low-abundance proteins such as cell surface proteins, organelle-specific proteins, and post-translational protein modifications such as phosphorylation or glycosylation.
Related products SILAC Protein Quantitation Kit (LysC), DMEM SILAC Protein Quantitation Kit (LysC), DMEM:F-12 SILAC Protein Quantitation Kit (LysC), RPMI-1640
For Research Use Only. Not for use in diagnostic procedures.
사양
배송 조건Approved for shipment on Wet or Dry Ice
수량1 kit
유형Protein Quantitation Kit
최종 제품 유형Protein
라벨 또는 염료Mass Isotope of Amino Acid
형식Kit
Unit Size1 kit
구성 및 보관
• DMEM for SILAC, 2 x 500 mL • 13C615N2 L-lysine-2 HCl, 1 x 50 mg • 13C615N4 L-arginine HCl, 1 x 50 mg • L-lysine-2 HCl, 1 x 50 mg • L-arginine-HCl, 1 x 50 mg, store at 4°C • 100 mL FBS, store (at -20°C)