B6-Puro Mouse Embryonic Fibroblasts, irradiated

Irradiated Gibco B6-Puro Mouse Embryonic Fibroblasts (MEFs) are puromycin-resistant feeder cells that support the culture of healthy undifferentiated human and mouse embryonic stem cells (ESCs) and induced pluripotent stem cells (iPSCs). Manufactured by MTI-GlobalStem and equivalent to MTI-GlobalStem Cat. No. GSC-6220G, these MEF cells are isolated from C57BL/6J-Tg(pPwL88puro)2EMS/J mice, mitotically arrested by irradiation, and tested to help ensure safety and performance. For other variants of drug-resistant MEFs, see CF6-Neo Mouse Embryonic Fibroblasts, Irradiated or DR4 Mouse Embryonic Fibroblasts, Irradiated. You may also choose from our selection of non-drug-resistant MEFs to suit your application.

• Help save precious time with pre-inactivated, ready-to-use MEFs
• Culture ESCs and iPSCs with confidence using meticulously tested feeder cells
• Perform drug selection with puromycin-resistant cells

Help save time with ready-to-use MEFs
Irradiated B6-Puro MEFs help save time and trouble dealing with an animal facility, performing tedious, time-consuming dissections, as well as expanding and testing cells day after day, week after week. Focus on your research with these pre-inactivated, ready-to-use feeders.

Culture with meticulously tested feeder cells
Each lot of B6-Puro MEFs is tested for bacteria, fungi, mycoplasma, and various mouse pathogens to minimize the risk of contamination. Each lot is also tested for post-thaw viability (typically greater than 95%) and ability to support mouse and human ES cells to ensure robust and consistent performance. These MEFs have successfully been used with ESC/iPSC lines from other species, but should be tested for compatibility if used for such an application.

Perform drug selection with puromycin-resistant cells
B6-Puro MEFs express puromycin N-acetyltransferase (pac) from a pPWL88puro transgene, making them highly resistant to puromycin. Each lot is tested for tolerance to various puromycin concentrations, and the threshold range for survival is reported in the certificate of analysis to aid in designing experiments. Use these feeder cells to support undifferentiated ESC or iPSC culture while performing puromycin selection.