The Ion Torrent Dual Barcode Kit 1-96 provides 96 unique dual-matched barcode adapters specifically designed to enable optimal performance of amplicon libraries and Ion semiconductor sequencers. When used in combination with Ion AmpliSeq and Oncomine assays, the kit enables users to pool up to 96 amplicon libraries to conduct multiplexed sequencing analysis, thereby increasing lab efficiency and reducing sequencing costs per sample. The barcode adapters are provided in 96-well plate format.

Features of the Ion Torrent Dual Barcode Kit 1-96 include:

• Enables multiplexing of numerous amplicon library samples on a single sequencing chip with the use of robust molecular barcodes to identify very rare clones with high accuracy
• Both sequence- and flow-optimized for equal representation of all barcodes in a pool and more economical multiplexed sequencing runs
• Robust error correction built in by design for added confidence in sample identification

Higher throughput and variant calling of rare clones

Multiplexing with dual-barcoded libraries enables cost-effective runs by permitting up to 96 samples per run, significantly decreasing the cost and handling requirements of sequencing. Each barcode was individually tested to minimize representation bias and provides superior sensitivity in detecting low-frequency clones with high accuracy.

Sequence- and flow-optimized for increased performance and efficiency

Barcode adapters contain sequences that are optimized to provide equal representation of all barcodes in a pool and require a minimal number of flows to interrogate, resulting in more economical multiplexed sequencing runs.

Robust error correction built in by design

Built-in error correction increases the accuracy of sample assignment.