The Invitrogen Colorimetric ReadiLAMP Kit for SARS-CoV-2 is a loop-mediated isothermal amplification (LAMP)-based solution for rapid SARS-CoV-2 detection in saliva and nasopharyngeal/nasal swab samples. This kit offers two protocol options. The first provides a workflow for RNA purification followed by a colorimetric LAMP assay. (For robust SARS-CoV-2 RNA isolation, the MagMAX Viral/Pathogen II Nucleic Acid Isolation Kit (Cat. No. A48383) or PureLink Viral RNA/DNA Mini Kit (Cat. No. 12280050) are recommended.) The second protocol consists of a simple SARS-CoV-2 viral lysis method for compatible sample types followed by a colorimetric LAMP assay. This second workflow does not require nucleic acid isolation or complex sample preparation; instead it directly incorporates the saliva or nasopharyngeal/nasal swab samples as LAMP assay input.

Features of the Colorimetric ReadiLAMP Kit for SARS-CoV-2 include:
• Simplicity—detection is fast, simple, and intuitive; LAMP reaction changes color from purple to blue if SARS target is present; no instrumentation required, except for a 65°C heating block
• Sensitivity—input of up to 50% volume of purified RNA per LAMP reaction, enabling maximal sensitivity in SARS-CoV-2 detection
    --Direct LAMP: 100–500 copies per reaction (25 μL)
    --Purified RNA-based LAMP: 20–100 copies per reaction (25 μL)
• Speed
    --Direct LAMP: ∼35 minutes using crude saliva or swabs in non-serum-containing transport medium
    --Purified RNA-based LAMP: ∼ 60 minutes (∼30 minutes RNA purification, 30 minutes LAMP assay)
• Throughput—process 1–96 samples at a time, enabling processing of more samples with same personnel headcount, using individual tubes, tube strips, or 96-well/384-well plates