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카탈로그 번호 | 제품 사이즈 | 제품 정가(KRW) | 재고 정보 | 수량 | |
---|---|---|---|---|---|
AM8439 | 50 µl | 제품 정가 322,000 온라인 행사 274,000 (01-Oct-2024에 종료) 공급가 | - |
When incorporated into DNA, Ambion 5-(3-aminoallyl)-dUTP provides a reactive group for addition of other chemical groups. The modified dUTP is readily incorporated by reverse transcriptase. The aminoallyl modification enables downstream reaction with amine-reactive compounds such as N-hydroxysuccinimide (NHS) esters; thus aminoallyl-modified cDNA can be labeled with any amine-reactive group moiety.
Use of this modified nucleotide
Use 5-(3-aminoallyl)-dUTP as recommended in your protocol for reverse transcription. Substitution of 30–80% of the dTTP with 5-(3-aminoallyl)-dUTP may be useful, but the exact proportion will depend on experimental goals.