색상 | Orange |
여기 파장 범위 | 570 nm |
염료 유형 | Cell-impermeant |
용도(애플리케이션) | Fluorescence Assays |
용도(장비) | Fluorescence Microscope |
수량 | 200 μL |
검출 방법 | Fluorescence |
설명 | BOBO™-3 Iodide (570/602), 1 mM solution in DMSO |
방출 | 602 nm |
형태 | Liquid |
제품라인 | BOBO™ |
배송 조건 | Room Temperature |
라벨 유형 | Fluorescent Dye |
제품 유형 | Nucleic Acid Stain |
SubCellular Localization | Nucleic Acids, Nucleus |
세포 투과성 | Cell-impermeant |
Unit Size | 200 µL |
색상 | Blue |
여기 파장 범위 | 462 nm |
염료 유형 | Cell-impermeant |
용도(애플리케이션) | Fluorescence Assays |
용도(장비) | Fluorescence Microscope |
수량 | 200 μL |
검출 방법 | Fluorescence |
설명 | BOBO™-1 Iodide (462/481), 1 mM solution in DMSO |
방출 | 481 nm |
형태 | Liquid |
제품라인 | BOBO™ |
배송 조건 | Room Temperature |
라벨 유형 | Fluorescent Dye |
제품 유형 | Nucleic Acid Stain |
SubCellular Localization | Nucleic Acids, Nucleus |
세포 투과성 | Cell-impermeant |
Unit Size | 200 µL |
색상 | Blue |
여기 파장 범위 | 434 nm |
염료 유형 | Cell-impermeant |
형식 | Tube |
용도(애플리케이션) | Fluorescent Labeling, Flow Cytometry |
용도(장비) | Fluorescence Microscope |
수량 | 200 μL |
검출 방법 | Fluorescence |
설명 | POPO™-1 Iodide (434/456), 1 mM Solution in DMSO |
방출 | 456 nm |
형태 | Liquid |
제품라인 | POPO™ |
배송 조건 | Room Temperature |
라벨 유형 | Fluorescent Dye |
제품 유형 | Nucleic Acid Stain |
SubCellular Localization | Nucleic Acids, Nucleus |
세포 투과성 | Cell-impermeant |
Unit Size | 200 µL |
색상 | Yellow |
여기 파장 범위 | 534 nm |
염료 유형 | Cell-impermeant |
형식 | Tube |
용도(애플리케이션) | Fluorescent Labeling, Flow Cytometry |
용도(장비) | Fluorescence Microscope |
수량 | 200 μL |
검출 방법 | Fluorescence |
설명 | POPO™-3 Iodide (534/570), 1 mM Solution in DMF |
방출 | 570 nm |
제품라인 | POPO™ |
배송 조건 | Room Temperature |
라벨 유형 | Fluorescent Dye |
제품 유형 | Nucleic Acid Stain |
SubCellular Localization | Nucleic Acids, Nucleus |
세포 투과성 | Cell-impermeant |
Unit Size | 200 µL |
색상 | Green |
여기 파장 범위 | 514 nm |
용도(애플리케이션) | Fluorescence Assays |
용도(장비) | Fluorescence Microscope |
수량 | 200 μL |
검출 방법 | Fluorescence |
설명 | TOTO™-1 Iodide (514/533), 1 mM Solution in DMSO |
방출 | 533 nm |
형태 | Liquid |
제품라인 | TOTO™ |
배송 조건 | Room Temperature |
라벨 유형 | Fluorescent Dye |
제품 유형 | Nucleic Acid Stain |
SubCellular Localization | Nucleic Acids, Nucleus |
세포 투과성 | Cell-impermeant |
Unit Size | 200 µL |
색상 | Red |
여기 파장 범위 | 642 nm |
용도(애플리케이션) | Fluorescence Assays |
용도(장비) | Fluorescence Microscope |
수량 | 200 μL |
검출 방법 | Fluorescence |
설명 | TOTO™-3 Iodide (642/660), 1 mM Solution in DMSO |
방출 | 660 nm |
형태 | Liquid |
제품라인 | TOTO™ |
배송 조건 | Room Temperature |
라벨 유형 | Fluorescent Dye |
제품 유형 | Nucleic Acid Stain |
SubCellular Localization | Nucleic Acids, Nucleus |
세포 투과성 | Cell-impermeant |
Unit Size | 200 µL |
색상 | Green |
여기 파장 범위 | 491 nm |
염료 유형 | Cell-impermeant |
형식 | Tube |
용도(애플리케이션) | Fluorescent Labeling |
용도(장비) | Fluorescence Microscope |
수량 | 200 μL |
검출 방법 | Fluorescence |
설명 | YOYO™-1 Iodide (491/509), 1 mM Solution in DMSO |
방출 | 509 nm |
제품라인 | YOYO™ |
배송 조건 | Room Temperature |
라벨 유형 | Fluorescent Dye |
제품 유형 | Nucleic Acid Stain |
SubCellular Localization | Nucleic Acids, Nucleus |
세포 투과성 | Cell-impermeant |
Unit Size | 200 µL |
색상 | Orange |
여기 파장 범위 | 612 nm |
염료 유형 | Cell-impermeant |
형식 | Tube |
용도(애플리케이션) | Fluorescent Labeling |
용도(장비) | Fluorescence Microscope |
수량 | 200 μL |
검출 방법 | Fluorescence |
설명 | YOYO™-3 Iodide (612/631), 1 mM Solution in DMSO |
방출 | 631 nm |
제품라인 | YOYO™ |
배송 조건 | Room Temperature |
라벨 유형 | Fluorescent Dye |
제품 유형 | Nucleic Acid Stain |
SubCellular Localization | Nucleic Acids, Nucleus |
세포 투과성 | Cell-impermeant |
Unit Size | 200 µL |
색상 | Orange, Green, Yellow, Red, Blue |
여기 파장 범위 | 436, 442, 488, 514, 543, 568, 594, 633 nm |
염료 유형 | Cell-impermeant |
형식 | Kit |
용도(애플리케이션) | Fluorescent Labeling |
용도(장비) | Fluorescence Microscope |
수량 | 25 μL |
검출 방법 | Fluorescence |
설명 | Nucleic Acid Stains Dimer Sampler Kit |
방출 | 456, 481, 509, 533, 570, 602, 631, 660 nm |
제품라인 | BOBO™, POPO™, TOTO™, YOYO™ |
배송 조건 | Room Temperature |
라벨 유형 | Fluorescent Dye |
제품 유형 | Stains Dimer Sampler Kit |
SubCellular Localization | Nucleic Acids |
세포 투과성 | Cell-impermeant |
Unit Size | 1 kit |
카탈로그 번호 | 사양 | 제품 사이즈 | 설명 | 제품 정가(KRW) | 재고 정보 | 수량 | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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B3586 | 200 µL | BOBO™-3 Iodide (570/602), 1 mM solution in DMSO | 제품 정가 982,000 온라인 행사 785,000 (01-Oct-2024에 종료) 공급가 | - | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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B3582 | 200 µL | BOBO™-1 Iodide (462/481), 1 mM solution in DMSO | 제품 정가 981,000 온라인 행사 784,000 (01-Oct-2024에 종료) 공급가 | - | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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P3580 | 200 µL | POPO™-1 Iodide (434/456), 1 mM Solution in DMSO | 제품 정가 1,050,000 온라인 행사 840,000 (01-Oct-2024에 종료) 공급가 | - | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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P3584 | 200 µL | POPO™-3 Iodide (534/570), 1 mM Solution in DMF | 제품 정가 938,000 온라인 행사 750,000 (01-Oct-2024에 종료) 공급가 | - | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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T3600 | 200 µL | TOTO™-1 Iodide (514/533), 1 mM Solution in DMSO | 제품 정가 960,000 온라인 행사 768,000 (01-Oct-2024에 종료) 공급가 | - | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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T3604 | 200 µL | TOTO™-3 Iodide (642/660), 1 mM Solution in DMSO | 제품 정가 1,089,000 온라인 행사 871,000 (01-Oct-2024에 종료) 공급가 | - | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Y3601 | 200 µL | YOYO™-1 Iodide (491/509), 1 mM Solution in DMSO | 제품 정가 1,003,000 온라인 행사 953,000 (01-Oct-2024에 종료) 공급가 | - | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Y3606 | 200 µL | YOYO™-3 Iodide (612/631), 1 mM Solution in DMSO | 제품 정가 969,000 온라인 행사 775,000 (01-Oct-2024에 종료) 공급가 | - | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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N7565 | 1 kit | Nucleic Acid Stains Dimer Sampler Kit | 제품 정가 669,000 온라인 행사 535,000 (01-Oct-2024에 종료) 공급가 | - | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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These eight spectrally distinct dimeric cyanine nucleic acid stains are among the highest sensitivity fluorescent probes available for nucleic acid staining. The carbocyanine dimers have very strong binding affinity for dsDNA, with dissociation constants in the micromolar range and nucleic acid-binding affinities greater than their parent monomer dyes. In addition to their high affinity for nucleic acids, cyanine dimers are essentially nonfluorescent in the absence of nucleic acids and exhibit 100- to 1000-fold fluorescence enhancements upon binding to DNA. The extinction coefficients and fluorescence quantum yields of the cyanine dimers bound to DNA are high, resulting in very bright fluorescence signals.
Features of the dimeric cyanine nucleic acid stains:
• High affinity—strong binding affinity for dsDNA with dissociation constants in the micromolar range
• Low background—nonfluorescent in the absence of nucleic acids
• Large fluorescence enhancement—100- to 1000-fold fluorescence enhancements upon binding to DNA
• Bright fluorescence—high extinction coefficients and fluorescence quantum yields resulting in very bright fluorescence signals
The dimeric cyanine dyes are useful for many applications including fluorescence microscopy and flow cytometry. These dyes are impermeant to viable cells and can be used as dead cell indictors. Their bright fluorescence signals and low backgrounds make them ideal for staining nucleic acids on solid supports, such as microarrays. They are also useful as nuclear and chromosome counterstains for multicolor fluorescence labeling experiments such as in immunohistochemistry (IHC) and immunocytochemistry (ICC). Prestaining nucleic acid samples prior to gel or capillary electrophoresis is also possible due to the extraordinary stability of the dye-nucleic acid complexes.
The dimeric cyanine nucleic acid stains are supplied in a unit size of 200 μL as 1 mM solutions in dimethylsulfoxide (DMSO) or dimethylformamide (DMF) for the POPO-3 stain. The fluorescence spectra of the eight dimeric cyanine nucleic acid stains cover the entire visible wavelength range and each dye differs in extinction coefficient and quantum yield.
POPO™-1 Iodide (434/456)
The blue-fluorescent POPO-1 stain has excitation/emission maxima ∼434/456 nm when bound to dsDNA in aqueous solution. It can be excited by ultraviolet (UV) excitation sources and the Hg arc (only) 436 nm laser.
BOBO™-1 Iodide (462/481)
The blue-fluorescent BOBO-1 stain has excitation/emission maxima ∼462/481 nm when bound to dsDNA in aqueous solution. It can be excited by ultraviolet (UV) excitation sources and the He-Cd 442 nm laser.
YOYO™-1 Iodide (491/509)
One of our highest affinity nucleic acid stains, the green-fluorescent YOYO-1 stain shows over 1000-fold increase in fluorescence when bound to dsDNA. It has excitation/emission maxima ∼491/509 nm when bound to nucleic acids and can be excited by ultraviolet (UV) excitation sources and the Ar 488 nm laser. YOYO-1 stain has proved extremely useful in the analysis of single molecules of DNA and is used in super-resolution microscopy applications such as STORM.
TOTO™-1 Iodide (514/533)
The green-fluorescent TOTO-1 stain has excitation/emission maxima ∼514/533 nm when bound to dsDNA in aqueous solution. The DNA-bound TOTO-1 dye also has a short-wavelength peak at ∼275 nm and can be excited by ultraviolet (UV) excitation sources and the Ar 514 nm laser.
POPO™-3 Iodide (534/570)
The yellow-fluorescent POPO-3 stain has excitation/emission maxima ∼534/570 nm when bound to dsDNA in aqueous solution. It can be excited by the He-Ne 543 nm laser.
BOBO™-3 Iodide (570/602)
The orange-fluorescent BOBO-3 stain has excitation/emission maxima ∼570/602 nm when bound to dsDNA in aqueous solution. It can be excited by the Kr 568 nm laser.
YOYO™-3 Iodide (612/631)
The orange-fluorescent YOYO-3 stain has excitation/emission maxima ∼612/631 nm when bound to dsDNA in aqueous solution. It can be excited by the He-Ne 594 nm laser.
TOTO™-3 Iodide (642/660)
The far-red fluorescent TOTO-3 stain has excitation/emission maxima ∼640/660 nm when bound to dsDNA in aqueous solution. It can be excited by the He-Ne 633 nm laser. The long-wavelength fluorescence of TOTO-3 stain is similar to Alexa Fluor 647 or Cy5 dyes and is well separated from that of commonly used fluorophores, such as Alexa Fluor dyes, Oregon Green, fluorescein (FITC), rhodamine (TRITC), Texas Red, coumarin (AMCA), Marina Blue, and Pacific Blue dyes. Additionally, long-wavelength light–absorbing dyes such as TOTO-3 stain have the advantage that their fluorescence is usually not obscured by the autofluorescence of tissues.
TOTO-3 stain has high selectivity for nuclear over cytoplasmic staining, making it useful as a nuclear counterstain and dead cell indicator, and is among the highest-sensitivity probes for nucleic acid detection. TOTO-3 stain gives strong and selective nuclear staining in cultured cells and in paraffin sections. Simultaneous labeling with a green-fluorescent SYTO dye and cell-impermeant TOTO-3 stain is frequently used to assess cell viability. TOTO-3 has a much higher extinction coefficient than DNA-bound propidium iodide and has been used for unique applications such as detecting microbial cells and microorganisms in soil environments.
Nucleic Acid Stains Dimer Sampler Kit
For researchers designing new applications, the Nucleic Acid Stains Dimer Sampler Kit provides samples of the eight spectrally distinct analogs of the dimeric cyanine dyes for testing. This kit contains 25 μL each of the following high-affinity nucleic acid stains based upon symmetric dimers of cyanine dyes:
• BOBO-1 iodide (Cat. No. B3582)
• BOBO-3 iodide (Cat. No. B3586)
• POPO-1 iodide (Cat. No. P3580)
• POPO-3 iodide (Cat. No. P3584)
• TOTO-1 iodide (Cat. No. T3600)
• TOTO-3 iodide (Cat. No. T3604)
• YOYO-1 iodide (Cat. No. Y3601)
• YOYO-3 iodide (Cat. No. Y3603)